We narrowed to 8,652 results for: Ott

1. pcDNA3.1(-)-EGFP-AP4-cyto

   Plasmid

   #229694

   Purpose

   Transient expression of EGFP AP4-cyto in mammalian cells

   Depositor

   Alpha Yap

   Article

   Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

   Insert

   EGFP; Control construct with tandem AP4 motifs (cytoplasm)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1(-)-EGFP-FP4-cyto

   Plasmid

   #229695

   Purpose

   Transient expression of FP4-cyto in mammalian cells

   Depositor

   Alpha Yap

   Article

   Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

   Insert

   EGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

   Plasmid

   #216421

   Purpose

   Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   3xmyc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPbU6_hdhfr/yfcu

   Plasmid

   #216422

   Purpose

   Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPV-C1-crRNA-cloning-EF1a-BA

   Plasmid

   #204623

   Purpose

   piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMC3-TPK2

   Plasmid

   #176180

   Purpose

   yeast MoClo level-0 part plasmid type 3 containing TPK2 gene

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   TPK2 (TPK2 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMC6-spacer

   Plasmid

   #176178

   Purpose

   yeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast marker

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   41 bp non-coding DNA spacer from pYTK048
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTH32 [pmyo-3::YFP-CaCyclOp(A-2x)::SL2::mCherry]

   Plasmid

   #168168

   Purpose

   Expression of YFP-CaCyclOp(A-2x) in BWMs of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

   Insert

   YFP-CaCyclOp(A-2x)
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   E497K and C566D

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTH42 [punc-17::YFP-CaCyclOp(A-2x)::SL2::mCherry]

   Plasmid

   #168171

   Purpose

   Expression of YFP-CaCyclOp(A-2x) in cholinergic motor neurons of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

   Insert

   YFP-CaCyclOp(A-2x)
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCTCON2-BXG-altTAG

    Plasmid

    #158132

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCTCON2-BXG-altTAG2

    Plasmid

    #158134

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the second serine residu…

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCTCON2-BXG-altTAG3

    Plasmid

    #158136

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the third alanine residu…

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCTCON2-BXG-altTAG4

    Plasmid

    #158137

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the third serine residue…

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG

    Plasmid

    #130684

    Purpose

    Expresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D453_F489del, E653_G1960del

    Promoter

    CMV

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG

    Plasmid

    #130683

    Purpose

    Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D409_E452del, E653_G1960del

    Promoter

    CMV

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-myc-Sema4A RNAiR

    Plasmid

    #115172

    Purpose

    expresses RNAi-resistant Sema4A with N-terminal myc tag

    Depositor

    Suzanne Paradis

    Article

    McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

    Insert

    Sema4A (Sema4a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Silent mutation between amino acids in ORF (401-4…

    Promoter

    CMV

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTIM

    Plasmid

    #81198

    Purpose

    pTIM in vitro transcription backbone plasmid with two multiple cloning sites and two Rho-independent transcriptional terminators.

    Depositor

    Andrés Vázquez-Torres

    Article

    Tapscott et al Sci Rep. 2018 Jun 21;8(1):9465. doi: 10.

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUAST YFP Rab8 DN

    Plasmid

    #58451

    Purpose

    expression of drosophila YFP Rab8 dominant negative mutant

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab8 (Rab8 Fly)
    

    Use

    Tags

    YFP

    Expression

    Insect

    Mutation

    T22N (dominant negative)

    Promoter

    GAL4

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pUAST YFP Rab2 DN

    Plasmid

    #58445

    Purpose

    expression of drosophila YFP-Rab2 dominant negative mutant

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab2 (Rab2 Fly)
    

    Use

    Tags

    YFP

    Expression

    Insect

    Mutation

    S20N (dominant negative)

    Promoter

    GAL4

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pUAST YFP Rab2 CA

    Plasmid

    #58443

    Purpose

    expression of drosophila YFP-Rab2 constitutively active mutant

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab2 (Rab2 Fly)
    

    Use

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q65L (constitutive active)

    Promoter

    GAL4

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only