Skip to main content
Addgene
Showing: 1561 - 1580 of 22521 results

  1. pBUN501

    Plasmid
    #50582
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    AtU6-26p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50582-plasmid-map-sequence-id-75642

    Enlarge

  2. pY036_ATP1A1_G3_Array

    Plasmid
    #86619
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86619-plasmid-map-sequence-id-168855

    Enlarge

  5. IL1RN gRNA1

    Plasmid
    #113130
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for gRNA expression, RSV for GFP expression
    Available since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    113130-plasmid-map-sequence-id-217998

    Enlarge

  9. pAAV-dSa-VPR

    Plasmid
    #99651
    Purpose
    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VP64-p65-RTA activator
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    99651-plasmid-map-sequence-id-191797

    Enlarge

  10. lenti-UCOE-dCas9-BFP-VP64

    Plasmid
    #196717
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196717-plasmid-map-sequence-id-388038

    Enlarge

  11. pcU6.3_gRNA_c1

    Plasmid
    #221381
    Purpose
    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1
    Depositor
    James Briscoe, Tatjana Sauka-Spengler
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    G. gallus U6.3
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221381-plasmid-map-sequence-id-454411

    Enlarge

  12. lenti-dCas9-VP64-BFP

    Plasmid
    #196711
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-VP64-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196711-plasmid-map-sequence-id-392561

    Enlarge

  13. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

    Plasmid
    #221449
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221449-plasmid-map-sequence-id-437431

    Enlarge

  14. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

    Plasmid
    #221450
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221450-plasmid-map-sequence-id-440139

    Enlarge

  15. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

    Plasmid
    #221451
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221451-plasmid-map-sequence-id-437432

    Enlarge

  16. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228551-plasmid-map-sequence-id-454860

    Enlarge

  17. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

    Enlarge

  18. pSpec5'

    Plasmid
    #228549
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228549-plasmid-map-sequence-id-454858

    Enlarge

  19. pSpec3'

    Plasmid
    #228548
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228548-plasmid-map-sequence-id-454857

    Enlarge

  20. pR6K-GFP-CASTIF

    Plasmid
    #199653
    Purpose
    R6K plasmid encoding a type I-F CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST I-F systems
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199653-plasmid-map-sequence-id-412253

    Enlarge

Showing: 1561 - 1580 of 22521 results