We narrowed to 17,269 results for: nans

1. pALOD4

   Plasmid

   #111026

   Purpose

   Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

   Depositor

   Arun Radhakrishnan

   Article

   Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

   Insert

   Domain 4 of Anthrolysin O
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: S404C, C472A

   Promoter

   T7

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NW11

   Plasmid

   #133443

   Purpose

   Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW11
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pST-KT

   Plasmid

   #44561

   Purpose

   for ectopic inducible expression of mycobacterial proteins

   Depositor

   Vinay Nandicoori

   Article

   Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

   Type

   Empty backbone

   Use

   E.coli-mycobacteria shuttle vector

   Tags

   Flag tag and His tag

   Expression

   Bacterial

   Mutation

   Promoter

   UV15 promoter

   Available since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. bRA89

   Plasmid

   #100950

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

   Depositor

   James Haber

   Article

   Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NW30

   Plasmid

   #133444

   Purpose

   Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW30
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pST-K

   Plasmid

   #44560

   Purpose

   for ectopic constitutive expression of mycobacterial proteins

   Depositor

   Vinay Nandicoori

   Article

   Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

   Type

   Empty backbone

   Use

   E.coli-mycobacteria shuttle vector

   Tags

   Flag tag and His tag

   Expression

   Bacterial

   Mutation

   Promoter

   UV15 promoter

   Available since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPR220.08

   Plasmid

   #78553

   Purpose

   Plasmid constitutively expressing phycocyanobilin (PCB) biosynthesis genes and UirS sensor kinase gene

   Depositor

   Jeffrey Tabor

   Article

   Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

   Insert

   uirS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   J23108

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMSP1E1D1

   Plasmid

   #161605

   Purpose

   Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009 . 464():211-31.

   Insert

   MSP1E1D1
   

   Use

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeat of h…

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NW50

   Plasmid

   #133445

   Purpose

   Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW50
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LgBit-NbALFA

    Plasmid

    #201484

    Purpose

    NanoBit detection of ALFA-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    LgBit-NbALFA
    

    Use

    Tags

    LgBit

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-HIS-Tol2

    Plasmid

    #112486

    Purpose

    functional recombinant Tol2 protein from E.coli

    Depositor

    Stephen Ekker

    Article

    Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

    Insert

    Tol2 Transposase (tpase Synthetic)
    

    Use

    Tags

    HIS/6x

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21a-hAIM2-GST

    Plasmid

    #51528

    Purpose

    Express GST-tagged hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21a-hAIM2-His6

    Plasmid

    #51532

    Purpose

    Express His6-tagged hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LgBit-NbV5

    Plasmid

    #201476

    Purpose

    NanoBit detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    LgBit-NbV5
    

    Use

    Tags

    LgBit

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHEA

    Plasmid

    #168297

    Purpose

    Bacterial surface display of polypeptides (nanobodies) in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

    Insert

    Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
    

    Use

    Tags

    6xHis, E-tag, and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NbAlfa-LgBit

    Plasmid

    #201483

    Purpose

    NanoBit detection of ALFA-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbALFA-LgBit
    

    Use

    Tags

    LgBit

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. bRA77

    Plasmid

    #100953

    Purpose

    GAL1 driven Cas9. gRNA can be cloned into BplI sites.

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. bRA90

    Plasmid

    #100951

    Purpose

    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LaM8-AK74-irFP

    Plasmid

    #159592

    Purpose

    Opto-Nanobody

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    LaM8 with AsLOV2 at AK74
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. B2088_BTB-IDR-FRB

    Plasmid

    #215101

    Purpose

    Expresses BCL6 BTB(1-129)-mCherry-FUS (IDR)-FRB in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    BTB-mCherry-FUS(IDR)-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge