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Plasmid#220592PurposeRetroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffoldDepositorInsertGot1 shRNA (Got1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-p31(comet)
Plasmid#221692PurposeMammalian expression of FLAG-tagged human p31(comet)DepositorInsertMAD2BP1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-p31(comet)-QF
Plasmid#221693PurposeMammalian expression of FLAG-tagged human p31(comet) (Q83A/F191A)DepositorInsertMAD2BP1
UseTagsExpressionMammalianMutationQ83A, F191APromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8∆R-MET15
Plasmid#207034PurposeExpression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.DepositorInsertATG8
UseTags3xHAExpressionYeastMutation∆RPromoterpATG8Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-4V5-Pho8-Met
Plasmid#207029PurposeExpression of 4V5-Pho8 under CPU1 promoter.DepositorInsertPho8
UseTags4xV5ExpressionYeastMutationPromoterpCUP1Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8∆GR-MET15
Plasmid#207033PurposeExpression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.DepositorInsertATG8
UseTags3xHAExpressionYeastMutation∆GRPromoterpATG8Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLuc/FLAG Metap2
Plasmid#209316PurposeLentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)DepositorInsertMETAP2 (METAP2 Human, human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLYCHOS/FLAG Metap2
Plasmid#209314PurposeLentiviral construct expressing FLAG Metap2 and shRNA against LYCHOSDepositorInsertLYCHOS (GPR155 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3 APPA
Plasmid#207124PurposeLentiviral plasmid to express mouse Mettl3 APPA mutantDepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HAExpressionMutationD395A and W398APromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MacMT3 Musa acuminata Metallothionein 3
Plasmid#200294PurposeBacterial expression plasmids for production of recombinant Musa acuminata Metallothionein 3DepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
TvMT Thermosynechococcus vulcanus Metallothionein
Plasmid#200299PurposeBacterial expression plasmids for production of recombinant Thermosynechococcus vulcanus MetallothioneinDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
XlMT Xenopus laevis Metallothionein
Plasmid#200293PurposeBacterial expression plasmids for production of recombinant Xenopus laevis MetallothioneinDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
PflQ2MT Pseudomonas fluorescens Q2-87 Metallothionein
Plasmid#200297PurposeBacterial expression plasmids for production of recombinant Pseudomonas fluorescens Q2-87 MetallothioneinDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
BcrMT1B Blue Crab Metallothionein 1B
Plasmid#200290PurposeBacterial expression plasmids for production of recombinant Blue Crab Callinectes sapidus Metallothionein 1BDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
LlMT Littorina littorea Metallothionein
Plasmid#200291PurposeBacterial expression plasmids for production of recombinant Littorina littorea MetallothioneinDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
OsMTI-1B Oryza sativa Metallothionein 1B
Plasmid#200295PurposeBacterial expression plasmids for production of recombinant Oryza sativa Metallothionein 1BDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein (LOC4332487 Oryza sativa)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mAmetrine1.1-WPRE
Plasmid#193333PurposeExpresses mAmetrine1.1 under the TRE promoter in mammalian cellsDepositorInsertmAmetrine1.1
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMETn-del-gRNA2
Plasmid#185007Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMETn-del-gRNA2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMETn_2
Plasmid#185019PurposeshRNA mediated knockdownDepositorInsertERV_MMETn
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMETn_1
Plasmid#185018PurposeshRNA mediated knockdownDepositorInsertERV_MMETn
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMETn-del-gRNA1
Plasmid#185006Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMETn-del-gRNA1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-GB1 Ile6Met
Plasmid#128687PurposeExpresses GB1 Ile6Met mutantDepositorInsertspg
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
His-TEV-GB1 Val29Met
Plasmid#128688PurposeExpresses GB1 Val29Met mutantDepositorInsertspg
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
His-TEV-GB1 Ala34Met
Plasmid#128689PurposeExpresses GB1 Ala34Met mutantDepositorInsertspg
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
His-TEV-GB1 Val39Met
Plasmid#128690PurposeExpresses GB1 Val39Met mutantDepositorInsertspg
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
His-TEV-GB1 Val54Met
Plasmid#128691PurposeExpresses GB1 Val54Met mutantDepositorInsertspg
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-GFAP104-ChromeT-mCherry
Plasmid#123126PurposeAAV-mediated expression of ChromeT-mCherry under the GFAP promoter.DepositorInsertChromeT-mCherry
UseAAVTagsmCherryExpressionMammalianMutationChannelrhodopsin-2 (ChR2) with mutations A71S/ E9…PromoterGFAP104Available sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 mitoAURKA Lys162Met
Plasmid#157752PurposeExpression of kinase-dead AURKA K62M in mitochondriaDepositorInsertAURKA (AURKA Human)
UseTagsExpressionMammalianMutationAURKA K162M is a kinase-dead version of AURKAPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p415 Met25 Glo3 ΔGRM-ΔC
Plasmid#129487PurposeExpression in S. cerevisiaeDepositorInsertGlo3 ΔGRM ΔC (GLO3 Budding Yeast)
UseTagsExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Met133I, M1381I, M186I ELL2
Plasmid#127263PurposeFor in vitro translation of human ELL2 with Met133I, M1381I, M186IDepositorInsertELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationThree Mets (133, 138, 186) to IleuPromoterT7Available sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChromeT-GFP
Plasmid#123316PurposeAAV-mediated expression of ChromeT-GFP under the Syn promoter.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianMutationChannelrhodopsin-2 (ChR2) with mutations A71S/ E9…PromoterSynAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met1 diUb (1-152, R42W)
Plasmid#110767PurposeBacterial expression of Met1 diUbDepositorInsertUBB (UBB Human)
UseTagsN-His6-3CExpressionBacterialMutationR42WPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
METTL3 shRNA 1 tet pLKO puro
Plasmid#162985PurposeTet-inducible shRNA targeting human METTL3 #1DepositorInsertmethyltransferase like 3 (METTL3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
METTL3 shRNA 2 tet pLKO puro
Plasmid#162984PurposeTet-inducible shRNA targeting human METTL3 #2DepositorInsertmethyltransferase like 3 (METTL3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDZ274 (pLEU MET25pro MCP-2x-yeGFP)
Plasmid#45929DepositorInsertMCP-2x-yeGFP
UseTags2x-yeGFPExpressionYeastMutationPromoterMET25Available sinceAug. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBRPyCAG-Mettl3-dsRed-IRES-puro
Plasmid#61516PurposeConstitutive expression of murine wild-type Mettl3DepositorInsertMettl3 (Mettl3 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGGAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSH100 (YCplac33 MET25pro MCP-mCherry)
Plasmid#45930DepositorInsertMCP-mCherry
UseTagsmCherryExpressionYeastMutationPromoterMet25Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)
Plasmid#115698PurposeER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiaeDepositorInsertSSKar2-sfpHluorin-HDEL
UseTagsER retention signal containing HDEL and ER signal…ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDZ276 (pURA MET28 PCP-2x-yeGFP)
Plasmid#35194DepositorInsertPCP-2x-yeGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-K27 histone methylation reporter
Plasmid#22865DepositorInsertK27 HMR
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only