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Plasmid#160440PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(0)-CRY2-mCherry
Plasmid#160439PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-PIF6-EGFP
Plasmid#154913Purpose1-100 amino acids of Arabidopsis PIF6 (Gene ID: 825382), tagged with EGFP fluorescent protein. Mammalian codon optimised.DepositorInsertphytochrome interacting factor 3-like 2 (PIL2 Mustard Weed)
UseXenopus/avian/zebrafishTagsEGFPExpressionMammalianMutation1-100 amino acids of Arabidopsis PIF6PromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIB-VP16 (B1014)
Plasmid#92036PurposeExpresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.DepositorInsertCIB1 (CIB1 Mustard Weed)
UseTagsFusion of plant CIB1 (intact NLS) with VP16 activ…ExpressionMammalianMutationPromoterT7Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CRY2-mCherry
Plasmid#140571PurposeFor use in light-induced protein inactivation through the interaction with CIB1DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMV IE94Available sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAL130
Plasmid#22475PurposeExpresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to PhyDepositorInsertPIF3NT (PIF3 Mouse, Mustard Weed)
UseSynthetic BiologyTagsmYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry PGK-1 in pcDNA3.1
Plasmid#64214PurposemCherry fused between Cry2 and PGK-1 to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry RGS4(del 1-33) in pcDNA3.1
Plasmid#64207PurposemCherry fused between Cry2 and RGS4(del 1-33) to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
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CIB-VP64 (B1016)
Plasmid#92037PurposeExpresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)DepositorInsertCIB1-VP64 (CIB1 Mustard Weed)
UseTagsFusion of plant CIB1 with VP64 activation domain.…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-CRY2-CreN
Plasmid#26888PurposeExpresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.DepositorUseTagsExpressionMammalianMutationaa 19-104 of CrePromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX
Plasmid#154910Purpose1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.DepositorInsertPhytochrome B (PHYB Mustard Weed, Synthetic)
UseXenopus/avian/zebrafishTagsCAAX membrane moiety and mCherryExpressionMammalianMutation1-621 amino acids of Arabidopsis PHYBPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Y276H PHYB-CAAX
Plasmid#154911Purpose1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.DepositorInsertPhytochrome B (PHYB Mustard Weed)
UseXenopus/avian/zebrafishTagsCAAX membrane moiety and mCherryExpressionMammalianMutation1-621 amino acids of Arabidopsis PHYB, Tyrosine r…PromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry GRK2 ct in pcDNA3.1
Plasmid#64212PurposemCherry fused between Cry2 and GRK2ct to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMV and cmvAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry GRK3 ct in pcDNA3.1
Plasmid#64213PurposemCherry fused between Cry2 and GRK3 ct to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mcherry Gamma 9 in pcDNA3.1
Plasmid#64208PurposemCherry fused between Cry2 and gamma 9 to study cell migrationDepositorUseTagsmcherry and n/aExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBTR(SMCytc)
Plasmid#72330PurposeExpresses spider monkey cytochrome c along with the heme lyase (CYC3) from yeast for heme insertionDepositorInsertspider monkey cytochrome c gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHSG+PTDH
Plasmid#66184Purposephosphite dehydrogenaseDepositorInsertPhosphite Dehydrogenase
UseUnspecifiedTagsExpressionMutationE175A, A176RPromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLRD09
Plasmid#208261PurposeProduction of biformene in E. coli MEV20DepositorInsertscldB
cldD
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-Rluc8
Plasmid#79844PurposeRluc8 reporter plasmidDepositorInsertRluc8
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only