We narrowed to 2,123 results for: D10

1. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

   Plasmid

   #98975

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 4 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98972

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

   Plasmid

   #98973

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 2 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKMD106h - intein-Cas9(C574)

   Plasmid

   #64191

   Purpose

   Expresses intein-Cas9(C574) in mammalian cells

   Depositor

   David Liu

   Article

   Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

   Insert

   mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
   

   Use

   CRISPR

   Tags

   NLS - 3x FLAG (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPD1019 TRE3GV DsRedExpress2 in TU2

   Plasmid

   #139254

   Purpose

   TRE3GV promoter and DsRedExpress2 in TUPV2

   Depositor

   Joshua Leonard

   Insert

   DsRedExpress2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHL-EF1a-SphcCas9(D10A)-iP-A

   Plasmid

   #60600

   Purpose

   Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Inserts

   CRISPR Cas9 D10A
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Changed Asp 10 to Ala, Codon usage optimized for …

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Flag-eSpCas9-D10A

   Plasmid

   #80449

   Purpose

   enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   ehSpCas9_D10A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-FLAG-mTUT4(D1028A)

   Plasmid

   #60220

   Purpose

   Mammalian expression of FLAG-tagged catalytic mutant of TUT4

   Depositor

   Zissimos Mourelatos

   Article

   Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

   Insert

   TUT4 (Tut4 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D1028A

   Promoter

   CMV

   Available since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pGEX6P1‐Afu‐rnhB D101N

   Plasmid

   #108695

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   D101N catalytic site mutation

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPD1025 EF1alpha TetON3G in TU6

    Plasmid

    #139262

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV6

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPD1026 EF1alpha TetON3G in TU2

    Plasmid

    #139258

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV2

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPD107 VP16-ZF43(RARR)

    Plasmid

    #138812

    Purpose

    Constitutive expression of VP16-ZF43(RARR) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF43(RARR)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R15A

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. hRad51(G151D)–Cas9(D10A)

    Plasmid

    #125565

    Purpose

    Expresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(G151D)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-GW1D10(aa566-1343)

    Plasmid

    #21517

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contain aa566-1343

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

15. hRad51(F86E)–Cas9(D10A)

    Plasmid

    #125564

    Purpose

    Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(F86E)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GST-GW1D10(aa566-1343)

    Plasmid

    #21516

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    contain aa566-1343

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

17. Top7 D9T D10V N14T

    Plasmid

    #12473

    Depositor

    David Baker

    Insert

    Top7 D9T D10V N14T
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D9T D10V N14T

    Promoter

    Available since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-nEF-SpCas9D10A nickase

    Plasmid

    #216736

    Purpose

    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    EF-1-alpha core

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid

    #199254

    Purpose

    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity S. pyogenes Cas9-D10A-KARA nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A R1060A

    Promoter

    CAG promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPD1024 EF1alpha TetON3G in TU5

    Plasmid

    #139261

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV5

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge