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We narrowed to 2,493 results for: D10

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Showing: 141 - 160 of 2493 results

  1. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140002-plasmid-map-sequence-id-275516

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  2. pcDNA3-Lyso-TORCAR

    Plasmid
    #64929
    Purpose
    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LAMP1-TORCAR (LAMP1 Human, Synthetic)
    Tags
    Cerulean, Full-length LAMP1, and YPet
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64929-plasmid-map-sequence-id-490376

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  3. PB_tre_dCas9_KRAB

    Plasmid
    #126030
    Purpose
    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion
    Depositor
    Mauro Calabrese
    Publication
    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9 fusion with KRAB
    Hygromycin resistance
    Use
    CRISPR; Piggybac
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive Cas9)
    Promoter
    EF1 alpha and TRE
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126030-plasmid-map-sequence-id-245793

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  8. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-477414

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  9. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Publication
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73796-plasmid-map-sequence-id-237125

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  10. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71685-plasmid-map-sequence-id-322543

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  11. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Publication
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83890-plasmid-map-sequence-id-240902

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  13. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74407-plasmid-map-sequence-id-140317

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  14. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

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  15. pcDNA3.1-CHRFAM7A-mCherry

    Plasmid
    #62635
    Purpose
    mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop
    Depositor
    Henry Lester
    Publication
    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHRFAM7A (CHRFAM7A Human)
    Expression
    Mammalian
    Mutation
    mCherry inserted into M3-M4 loop
    Promoter
    CMV
    Available since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  19. GoldenBraid 2.0 Kit

    Plasmid kit
    #1000000076
    Purpose
    Plant synthetic biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts.
    Depositor
    Diego Orzaez
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Plant Expression
    Editing type
    CRISPR
    Cloning type
    Golden Gate (GoldenBraid)
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pcDNA3.1-CHRFAM7ADelta2bp

    Plasmid
    #62515
    Purpose
    mammalian expression of human duplicated Alpha7 with 2 bp deletion (CHRFAM7A-∆2bp)
    Depositor
    Sherry Leonard, Henry Lester
    Publication
    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHRFAM7ADelta2bp (CHRFAM7A Human)
    Expression
    Mammalian
    Mutation
    partially duplication of CHRNA7 with 2-bp deletio…
    Promoter
    CMV
    Available since
    March 20, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 141 - 160 of 2493 results