Skip to main content
Addgene
Showing: 141 - 160 of 2384 results

  7. pGG198

    Plasmid
    #165605
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Secondary protospacer ('CGGCG' PAM) ins…
    Promoter
    lac
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    165605-plasmid-map-sequence-id-338038

    Enlarge

  9. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

    Plasmid
    #154196
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC1-A, sgRNA-BC1-C
    Use
    Lentiviral; Catch barcode activation
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and mU6
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154196-plasmid-map-sequence-id-308676

    Enlarge

  11. Ad:2CMV_A2R2-N25Ctr

    Plasmid
    #122243
    Purpose
    Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Corey Neu
    Article
    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516
    Inserts
    C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Mutation
    deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…
    Promoter
    CMV
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    122243-plasmid-map-sequence-id-239188

    Enlarge

  12. pENTR223-CMV-Cre-U6-sgNotch1#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid
    #209068
    Purpose
    Entry vector that encodes sgRNAs against mouse Notch1, Rb1, Trp53, Rbl2, and CMV Cre recombinase.
    Depositor
    Matthew Oser
    Article
    Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d
    Insert
    sgRNAs targeting Notch1, Rb1, Trp53, Rbl2 and Cre recombinase
    Use
    Gateway vector to be used for lr reaction
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    209068-plasmid-map-sequence-id-412279

    Enlarge

  13. hNTa2-qgRNA-pYJA5

    Plasmid
    #217780
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217780-plasmid-map-sequence-id-469426

    Enlarge

  14. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide4

    Plasmid
    #118180
    Purpose
    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118180-plasmid-map-sequence-id-240371

    Enlarge

  15. pAAV CAG Barcode7

    Plasmid
    #229069
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    CAG Barcode7
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    229069-plasmid-map-sequence-id-455092

    Enlarge

  16. pAAV Syn Barcode21

    Plasmid
    #226194
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode21
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226194-plasmid-map-sequence-id-447882

    Enlarge

  17. CaV1.3e[8a,11,31b,Δ32,Δ42a]

    Plasmid
    #49332
    Purpose
    Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
    Depositor
    Diane Lipscombe
    Article
    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.
    Insert
    CACNA1D (Cacna1d Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains exons 8a, 11, and 31b.Exons 32 and 42a a…
    Promoter
    CMV
    Available since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    49332-plasmid-map-sequence-id-215242

    Enlarge

  19. mU6-sgNT-hU6-sgLkb1_2nd

    Plasmid
    #177223
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNT1/sgLkb1_2nd
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177223-plasmid-map-sequence-id-351959

    Enlarge

  20. mU6-sgLkb1_2nd-hU6-sgNT

    Plasmid
    #177222
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_2nd/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    177222-plasmid-map-sequence-id-350113

    Enlarge

Showing: 141 - 160 of 2384 results