We narrowed to 197 results for: single guide rna sgrna

1. pJR89

   Plasmid

   #140096

   Purpose

   sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pgRNAtet-phaZ

   Plasmid

   #169874

   Purpose

   Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   sgRNA towards phaZ in Pseudomonas putida
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pgRNAkan-glpR

   Plasmid

   #169873

   Purpose

   Single guide RNA plasmid for Cas9 targeting glpR in P. putida

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   sgRNA towards glpR in Pseudomonas putida
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. rtTA-sg-mGreenLantern332

   Plasmid

   #188483

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern sgRNA
   

   Use

   CRISPR

   Tags

   PB_rtTA_BsmBI

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. rtTA-sg-CMV152

   Plasmid

   #188485

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. rtTA-sg-CMV162

   Plasmid

   #188486

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. rtTA-sg-CMV51

   Plasmid

   #188484

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX459-sg-CMV152

   Plasmid

   #179916

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX459-sg-CMV51

   Plasmid

   #179915

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX459-sg-CMV162

    Plasmid

    #179917

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX459-sg-mGreenLantern332

    Plasmid

    #179914

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEE491

    Plasmid

    #149292

    Purpose

    T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEE531

    Plasmid

    #149290

    Purpose

    T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEE495

    Plasmid

    #149291

    Purpose

    T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX459-sg-tdTomato90/816

    Plasmid

    #179918

    Purpose

    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX459-sg-tdTomato166/892

    Plasmid

    #179919

    Purpose

    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDECKO_mCherry

    Plasmid

    #78534

    Purpose

    Backbone to clone single or paired sgRNAs

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA)

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEE391

    Plasmid

    #149281

    Purpose

    T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbPDS3sgRNA_FT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEE392

    Plasmid

    #149282

    Purpose

    T-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbPDS3sgRNA_mFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only