We narrowed to 3,495 results for: ttl

1. pF708-pET28a-Hs-delta38-METTL20-NHis wt

   Plasmid

   #85109

   Purpose

   expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta38) METTL20 (METTL20 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pShuttle-EGFP-CLASP2 340-1084

   Plasmid

   #24383

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1084) (CLASP2 Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CLASP2 deletion mutant that retains MT lattice bi…

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pShuttle-EGFP-CLASP2 340-1362 9xS/A

   Plasmid

   #24373

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1362) 9xS/A (CLASP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant. M…

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen

   Plasmid

   #207123

   Purpose

   Lentiviral vector to express human METTL3

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   hMETTL3 (METTL3 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-C-Myc-DDK-IRES-Puro METTL3

   Plasmid

   #200724

   Purpose

   Lentiviral expression of Flag-Tagged human METTL3

   Depositor

   Claudio Alarcón

   Insert

   METTL3 (METTL3 Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShuttle-EGFP-CLASP2 340-1084 9xS/A

   Plasmid

   #24384

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1084) 9xS/A (CLASP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant. M…

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector

   Plasmid

   #182346

   Purpose

   PacI-flanked CAG-LSL expression cassette with a FNF for positive selection

   Depositor

   Mario Capecchi

   Insert

   tdTomato
   

   Use

   Mouse Targeting; Flp / frt

   Expression

   Mammalian

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

   Plasmid

   #207136

   Purpose

   DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9_CL

   Plasmid

   #166245

   Purpose

   regulated dCas9 generator

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

   Insert

   P112-sgRNA-term-P104-RBS1-dCas9-B15
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ec-TTL-P112 and Ec-TTL-P104

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. BJ5183 cells

    Bacterial Strain

    #16398

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Bacterial Resistance

    Streptomycin

    Species

    Escherichia coli

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pAAV.GFAP.SF-iGluSnFR.A184S (AAV1)

    Viral Prep

    #106192-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.GFAP.SF-iGluSnFR.A184S (#106192). In addition to the viral particles, you will also receive purified pAAV.GFAP.SF-iGluSnFR.A184S plasmid DNA. GFAP-driven expression of SF-iGluSnFR.A184S. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Promoter

    GFAP

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

12. pAAV.hSynapsin.SF-iGluSnFR.A184S (AAV1)

    Viral Prep

    #106174-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSynapsin.SF-iGluSnFR.A184S (#106174). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.A184S plasmid DNA. hSynapsin driven SF-iGluSnFR.A184S, glutamate sensor. The A184S mutant has a higher glutamate affinity. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Promoter

    Syn

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pAAV.hSynapsin.SF-iGluSnFR.A184S (AAV9)

    Viral Prep

    #106174-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.hSynapsin.SF-iGluSnFR.A184S (#106174). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.A184S plasmid DNA. hSynapsin driven SF-iGluSnFR.A184S glutamate sensor. The A184S mutant has a higher glutamate affinity. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Promoter

    Syn

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

14. pAAV.hSynap-FLEX.SF-iGluSnFR.A184S (AAV5)

    Viral Prep

    #106180-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.hSynap-FLEX.SF-iGluSnFR.A184S (#106180). In addition to the viral particles, you will also receive purified pAAV.hSynap-FLEX.SF-iGluSnFR.A184S plasmid DNA. Synapsin-driven, Cre-dependent expression of glutamate sensor iGluSnFR (high affinity). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Promoter

    Syn

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S (AAV1)

    Viral Prep

    #106183-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S (#106183). In addition to the viral particles, you will also receive purified pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S plasmid DNA. Synapsin-driven, Cre-dependent SF-Venus-iGluSnFR.A184S glutamate sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Promoter

    Syn

    Tags

    Venus (Cre-dependent)

    Available Since

    Dec. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pAAV.hSynapsin.SF-iGluSnFR.S72A (AAV1)

    Viral Prep

    #106176-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSynapsin.SF-iGluSnFR.S72A (#106176). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.S72A plasmid DNA. hSynapsin driven SF-iGluSnFR.S72A, glutamate sensor. The S72A mutant has lower affinity for glutamate. Slower on-rate and faster off-rate. Enhanced temporal resolution and spatial resolution. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Promoter

    Syn

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

17. pAAV.hSynap-FLEX.SF-iGluSnFR.A184V (AAV1)

    Viral Prep

    #106181-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSynap-FLEX.SF-iGluSnFR.A184V (#106181). In addition to the viral particles, you will also receive purified pAAV.hSynap-FLEX.SF-iGluSnFR.A184V plasmid DNA. Synapsin-driven, Cre-dependent expression of glutamate sensor iGluSnFR (medium affinity). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Promoter

    Syn

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A (AAV1)

    Viral Prep

    #106185-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A (#106185). In addition to the viral particles, you will also receive purified pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A plasmid DNA. Synapsin-driven, Cre-dependent SF-Venus-iGluSnFR.S72A glutamate sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Promoter

    Syn

    Tags

    Venus (Cre-dependent)

    Available Since

    Dec. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pAUX_OL

    Plasmid

    #166246

    Purpose

    use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
    

    Expression

    Bacterial

    Promoter

    Ec-TTL-P112 and BBa_J23116

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH29_32 series plasmid

    Plasmid

    #120901

    Purpose

    Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

    Inserts

    Ec-TTL-P109
    sRNA-A targeting sequence
    RBS TIR=974 of GFPop1 gene
    GFPop1
    RBS TIR=6474 of ECF32mut gene
    ECF32mut
    T7 terminator
    resource competitor
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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