We narrowed to 2,891 results for: 974

1. pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS

   Plasmid

   #199353

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-TEV

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEND-192_Cacnes_HS

   Plasmid

   #225621

   Purpose

   C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   HspR
   

   Use

   Synthetic Biology

   Tags

   pFAST-6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. EYFP-Mito-7

   Plasmid

   #56596

   Purpose

   Localization: Mitochondria, Excitation: 513, Emission: 527

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Cathepsin S S21C/C25A

   Plasmid

   #84695

   Purpose

   for expression of mutant Cathepsin S in Leishmania tarantolae

   Depositor

   Hans Brandstetter

   Article

   Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

   Insert

   cathepsin S (CTSS Human)
   

   Use

   Expression in leishmania tarantolae

   Tags

   his-tag

   Expression

   Mutation

   S21C, C25A

   Promoter

   unknown

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mito-mEosEM-E

   Plasmid

   #226335

   Purpose

   Photoswitchable fluorescent protein for correlative light-electron microscopy; mitochondria-targeted mEosEM-E for mammalian expression

   Depositor

   Pingyong Xu

   Article

   Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

   Insert

   COX8A (COX8A Human)
   

   Use

   Tags

   mEosEM-E

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199367

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-SUMOEu-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry Codon 59 sgRNA

   Plasmid

   #109431

   Purpose

   MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry codon 59 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-Flag CASTOR1 I280A

   Plasmid

   #84495

   Purpose

   overexpression of CASTOR1 I280A mutant

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 I280A (GATSL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   I280A

   Promoter

   CMV

   Available since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pTS116 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199369

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-SUMOstar-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-flag Mst2 (KR)

    Plasmid

    #27372

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    Mst2 (STK3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    K56R (catalytically inactive)

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRGEB-Cas9-VirD2 (PCO)

    Plasmid

    #139802

    Purpose

    Expressing Cas9-VirD fusion under ubiquitin promoter

    Depositor

    Magdy Mahfouz

    Article

    Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

    Insert

    Cas9-VirD2
    

    Use

    Tags

    Cas9-VirD2

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Flag-YAP-S109A

    Plasmid

    #33088

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S109A

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

    Plasmid

    #199355

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA-SUMOstar

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199365

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5 FRT TO myc hSeparase

    Plasmid

    #59820

    Purpose

    Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-mGold-Mito-N-7

    Plasmid

    #158007

    Purpose

    Visualization of mitochondria in mammalian cells using mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEBB COMMD5 Flag

    Plasmid

    #74897

    Purpose

    Express COMMD5 in mammalian cells

    Depositor

    Colin Duckett, Ezra Burstein

    Article

    Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

    Insert

    COMMD5 (COMMD5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYLL204

    Plasmid

    #53455

    Purpose

    Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)

    Depositor

    Lisa Lyu, Leroy Liu

    Article

    Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

    Insert

    HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y814F

    Promoter

    CMV

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98972

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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