We narrowed to 3,526 results for: mpo

1. [UBC][firefly luciferase][target cloning][renilla luciferase]

   Plasmid

   #97190

   Purpose

   Reporter for translation activation via Pum.

   Depositor

   Edward Boyden

   Article

   Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Inserts

   Firefly Luciferase
   Renilla Luciferase
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Mammalian

   Promoter

   UBC (ubiquitin C)

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 10XSTAT92E–luciferase

   Plasmid

   #37393

   Depositor

   Norbert Perrimon

   Article

   Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

   Insert

   SOCS36E enhancer fragment (Socs36E Fly)
   

   Use

   Luciferase; Jak/stat reporter construct

   Expression

   Insect

   Promoter

   Hsp70

   Available Since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-6P-1-HsRCK_E

   Plasmid

   #146210

   Purpose

   Bacterial Expression of HsRck

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   HsRck (DDX6 Human)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pConRef-2H12

   Plasmid

   #221182

   Purpose

   cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen
   mScarlet-I
   

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen)

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-PKCa-mEGFP-N1

   Plasmid

   #112269

   Purpose

   IDOCKSalpha Donor Construct

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   Protein Kinase C alpha (PRKCA Bovine)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   A11V (please see depositor comments below)

   Promoter

   CMV

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. opto-Itsn1

   Plasmid

   #174509

   Purpose

   Expresses opto-Itsn1 (Itsn1DH-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   opto-Itsn1-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-(SIM)3

   Plasmid

   #126955

   Purpose

   Bacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
   

   Tags

   eGFP

   Expression

   Bacterial

   Promoter

   unknown

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-GfaABC1D-GRAB_eCB2.0

   Plasmid

   #164609

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   AAV

   Mutation

   S383T

   Promoter

   GfaABC1D

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-PuroR Xlone eGFP

   Plasmid

   #179837

   Purpose

   Doxycycline-inducible expression of eGFP

   Depositor

   Xiaoping Bao

   Article

   Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

   Insert

   eGFP
   

   Use

   CRISPR, Synthetic Biology, and TALEN ; Donor plas…

   Expression

   Mammalian

   Promoter

   TRE3GS

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDN80

    Plasmid

    #61349

    Purpose

    LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Type

    Empty backbone

    Tags

    AsLOV2 wt and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAD-CMV-STAT5A-CMV-GFP

    Plasmid

    #83251

    Purpose

    Adenoviral expression of STAT5A with co-expression of GFP

    Depositor

    Andrew Brooks

    Article

    Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

    Inserts

    Stat5a (Stat5a Mouse)
    eGFP
    

    Use

    Adenoviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-ChRmine-mRuby2-ST

    Plasmid

    #227777

    Purpose

    Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChRmine-mRuby2-ST
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSR62

    Plasmid

    #125076

    Purpose

    Expression of ompR under PLtetO-1, output promoter PompF146

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR (ompR )
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMGIB-3xFLAG-reptin

    Plasmid

    #53609

    Purpose

    Retroviral expression of human reptin

    Depositor

    Steven Artandi

    Article

    Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

    Insert

    reptin (RUVBL2 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    CDS mutated to confer resistance to shRNA. All mu…

    Promoter

    LTR

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pLYS1-EMRE/RNAi resistant

    Plasmid

    #50056

    Purpose

    Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    EMRE-RNAi resistant (SMDT1 Human)
    

    Expression

    Mammalian

    Mutation

    RNAi resistant, silent mutant

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDB22

    Plasmid

    #61342

    Purpose

    Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Tags

    GS linker-AsLov2-cMyc1A NLS and PKIt NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMIR-His-Halo-Tev-PTEN

    Plasmid

    #58241

    Purpose

    expresses halo tagged PTEN protein

    Depositor

    Yimon Aye

    Article

    Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

    Insert

    phosphatase and tensin homolog (PTEN Human)
    

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAD-CMV-STAT5B-CMV-GFP

    Plasmid

    #83257

    Purpose

    Adenoviral expression of STAT5B with co-expression of GFP

    Depositor

    Andrew Brooks

    Article

    Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

    Inserts

    Stat5b (Stat5b Mouse)
    eGFP
    

    Use

    Adenoviral

    Promoter

    CMV

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330 EGFR-sgRNA

    Plasmid

    #188633

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Dlx-FLEX-hM4Di-mCherry

    Plasmid

    #209701

    Purpose

    Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

    Depositor

    Peer Wulff

    Article

    Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    mCherry

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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