We narrowed to 5,560 results for: pcas

1. pCAG-T7-TALEN(Sangamo)-Destination

   Plasmid

   #37184

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Use

   Tags

   FokI nuclease

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Cre-T2A-mRuby2

   Plasmid

   #102989

   Purpose

   Expresses Cre and mRuby2

   Depositor

   Karin Scharffetter-Kochanek

   Article

   Meyer et al PLoS Comput Biol. 2017 Dec 4;13(12):e100

   Inserts

   Cre (site-specific recombinase)
   mRuby2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKRG3-mU6-PUb-hSpCas9

   Plasmid

   #162162

   Purpose

   Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   N/A

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-hChR2(H134R)-EYFP

   Plasmid

   #114367

   Purpose

   expresses channelrhodopsin 2 fused with EYFP

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   hChR2(H134R)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   the Histidine at amino acid position 134 was chan…

   Promoter

   pCAG

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T-CAG-SpCas9-BlastR

   Plasmid

   #107190

   Purpose

   Confers constitutive expression of SpCas9

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   SpCas9 (NEWENTRY )
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Chicken ß-actin

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-mTagBFP2-T2A-iCre (v2)

   Plasmid

   #125822

   Purpose

   Monomeric TagBFP2 and iCre recombinase (version 2)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Cas13d-T2A-Citrine

   Plasmid

   #224565

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   Cas13d-T2A-Citrine
   

   Use

   CRISPR

   Tags

   T2A-Citrine

   Expression

   Mutation

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-RFP-p2a-postASAP

   Plasmid

   #178793

   Purpose

   Expresses voltage sensor targeted to dendrites and spines along with RFP

   Depositor

   Rafael Yuste

   Article

   Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

   Insert

   FingR.PSD95, ASAP2f and TagRFP
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   mutations in amino acids L146G, S147T N149R, S150…

   Promoter

   CAG

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-sfGFP-iCre-ERT2

   Plasmid

   #125749

   Purpose

   sfGFP and iCreERT2 fused by unskippable linker

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   sfGFP-iCre-ERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-scFvGCN4sfGFP-VP64-GB1

    Plasmid

    #141417

    Purpose

    Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

    Depositor

    Izuho Hatada

    Article

    Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

    Insert

    scFvGCN4sfGFP-VP64-GB1
    

    Use

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-pac-T2A-iCre (v2)

    Plasmid

    #125821

    Purpose

    Puromycin resistance gene and iCre recombinase (version 2)

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS/ES-EnvA-VSVg-WPRE-pA

    Plasmid

    #87221

    Purpose

    to package wild type EnvA psudotyped lentivirus

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    EnvA-VSVg
    

    Use

    Mouse Targeting and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-OSF-PP-human-CHMP3

    Plasmid

    #154180

    Purpose

    expresses human CHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (CHMP3 Human)
    

    Use

    Tags

    FLAG and Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGG>nls-hCas9-nls

    Plasmid

    #99138

    Purpose

    Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    humanized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS D614G soluble Spike

    Plasmid

    #164651

    Purpose

    Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 soluble spike (S SARS-CoV-2)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CAG

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

16. pCAG-VSFP Butterfly 1.2

    Plasmid

    #47978

    Purpose

    Genetically encoded voltage indicator

    Depositor

    Thomas Knopfel

    Article

    Akemann et al J Neurophysiol. 2012 Oct;108(8):2323-37.

    Insert

    VSFP Butterfly 1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-SpCas9D10A nickase

    Plasmid

    #216737

    Purpose

    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMVd1

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174840

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    ENO1p

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS D614 soluble Spike

    Plasmid

    #164652

    Purpose

    Expresses SARS-CoV-2 soluble Spike protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 soluble spike (S SARS-CoV-2)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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