We narrowed to 26,366 results for: vit;

1. pcDNA3-ExRai-AktAR

   Plasmid

   #118462

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AktAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNAintron-XKR8-n3xFLAG

   Plasmid

   #215693

   Purpose

   Produces the XKR8 lipid scramblase with an n-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

   Insert

   XKR8-n3xFLAG (XKR8 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. tetO-NKX2.5

   Plasmid

   #46029

   Depositor

   John Gearhart

   Article

   Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

   Insert

   NKX2.5 (NKX2-5 Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-PKAKTRClover

   Plasmid

   #59146

   Purpose

   ORF encoding PKA KTR mClover flanked by gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   PKA Kinase Translocation Reporter (PRKACA Mouse, Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-blueCKAR

   Plasmid

   #118472

   Purpose

   cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueCKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA24N-ligase-cTF

   Plasmid

   #87743

   Purpose

   IPTG-inducible expression of ligase-cTF fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and cTF (chimeric transcription factor base…

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB-LIC-Bse

   Plasmid

   #26108

   Purpose

   SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   His6 - TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available Since

   Oct. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. RC(WW)-LgBit

   Plasmid

   #208931

   Purpose

   Recognition domain for in vitro MAPK-activity sensor

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   WW domain of PIN1 (PIN1 Human)
   

   Use

   Tags

   HISx6, LgBit, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-ExRai-AKAR-NES

   Plasmid

   #118407

   Purpose

   Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR-NES
   

   Use

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ROZA-XL

    Plasmid

    #64194

    Purpose

    Fluorescent reporter for ZAP-70 tyrosine kinase activity

    Depositor

    Annemarie Lellouch

    Article

    Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.

    Insert

    ROZA-XL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ROZA-XL

    Promoter

    CMV

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pDNA3-Cerulean-FHA1-NES

    Plasmid

    #138202

    Purpose

    Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    Cerulean-FHA1-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-PKCsub-YPet-NES

    Plasmid

    #138207

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub-YPet-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-ExRai-AKAR-Kras

    Plasmid

    #118408

    Purpose

    Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR-Kras
    

    Use

    Tags

    C-terminal targeting sequence from Kras

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-JNKAR1-NES

    Plasmid

    #84632

    Purpose

    Unimolecular, FRET-based JNK activity reporter; cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ECFP-FHA1-JNKsubDD-Citrine-NES
    

    Use

    Tags

    6xHis, Citrine, ECFP, Nuclear export signal (NES)…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMtb CblA

    Plasmid

    #138298

    Purpose

    Expression of Mtb CblA

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Insert

    Mtb CblA
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMtb ATR

    Plasmid

    #138297

    Purpose

    Expression of Mtb ATR

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Insert

    Corrinoid adenosyl transferase
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Cerulean-WW-NES

    Plasmid

    #84629

    Purpose

    Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    Cerulean-WW-NES
    

    Use

    Tags

    6xHis, Cerulean, Nuclear export signal (NES), T7 …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJOE5751.1

    Plasmid

    #135083

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-His tag-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-JNKsubDD-YPet-NES

    Plasmid

    #84633

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    JNKsubDD-YPet-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-PKAsub-YPet-NES

    Plasmid

    #138203

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub-YPet-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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