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R979-X57-008: Hs.KRAS4b(1-169)

Plasmid

#159690

Purpose

Baculovirus protein expression of KRAS4b (1-169)

Depositor

Dominic Esposito

Article

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-169) (KRAS Human)

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZNF597_WT

Plasmid

#82894

Purpose

Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZNF597 (ZNF597 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET24c(+)-EFTu

Plasmid

#31172

Depositor

Linda Spremulli

Article

Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

Insert

E. coli elongation factor Tu (tufB E. coli)

Tags

His

Expression

Bacterial

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pCB6-X:31HA

Plasmid

#74371

Purpose

Mammalian expression of wild-type HA from the X:31 strain of influenza virus

Depositor

Judith White

Article

Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.

Insert

HA

Expression

Mammalian

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pCMV4-Tax(M20)

Plasmid

#23285

Depositor

Warner Greene

Article

Smith et al Genes Dev. 1990 Nov . 4(11):1875-85.

Insert

Tax(M20)

Expression

Mammalian

Mutation

T130A L131S

Available Since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
pOL2339

Plasmid

#37839

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Ub-V

Tags

mCherry

Expression

Worm

Promoter

nhx-2

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pMAM-RecG-EGFP

Plasmid

#31274

Depositor

Andrew Pierce

Article

Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

Insert

RecG-EGFP

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pKS2236

Plasmid

#37833

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

YFP

Tags

YFP

Expression

Worm

Promoter

nhx-2

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only

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PM-TD!TB

Plasmid

#48656

Purpose

Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-NM!TA

Plasmid

#48651

Purpose

Bacterial NM crRNA expression: targets NM to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-TD!TA

Plasmid

#48655

Purpose

Bacterial TD crRNA expression: targets TD to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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G1S-HA-pCB6

Plasmid

#74373

Purpose

Mammalian expression of a G1S mutant HA from the X:31 strain of influenza virus

Depositor

Judith White

Article

Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.

Insert

HA

Expression

Mammalian

Mutation

G1S

Promoter

CMV

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
G1V-HA-pCB6

Plasmid

#74372

Purpose

Mammalian expression of a G1V mutant HA from the X:31 strain of influenza virus

Depositor

Judith White

Article

Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.

Insert

HA

Expression

Mammalian

Mutation

G1V

Promoter

CMV

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
PM-NM!TB

Plasmid

#48652

Purpose

Bacterial NM crRNA expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-SP!TA

Plasmid

#48649

Purpose

Bacterial SP crRNA expression: targets SP to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pSKA322

Plasmid

#101676

Purpose

empty backbone with chloramphenicol resistance

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHA1

Plasmid

#85709

Purpose

This plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

Depositor

Ulrich Koszinowski, Martin Messerle

Article

Adler et al J Virol. 2000 Aug;74(15):6964-74.

Type

Empty backbone

Use

Cre/Lox; Herpesvirus cloning

Expression

Bacterial

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSAM_Cam

Plasmid

#91570

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

Depositor

Harry Mobley

Article

Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only
pVHD

Plasmid

#61303

Purpose

Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tag

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

Type

Empty backbone

Tags

HA

Expression

Bacterial

Promoter

PV10

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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