Addgene

We narrowed to 2,456 results for: Kap

Showing: 1601 - 1620 of 2456 results

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pTetON-DeAct-GS1

Plasmid

#89454

Purpose

Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-GS1 (GSN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning cytoplasmic Gsn amino acids 5…

Promoter

TRE3G

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLE1

Plasmid

#160802

Purpose

Express POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLE1 (POLE Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mutation

shPOLE_1 and shPOLE2 resistant

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Trim46

Plasmid

#176402

Purpose

TRIM46 tagged with GFP

Depositor

Thomas Schwarz, Casper Hoogenraad

Article

van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

Insert

trim46 (Trim46 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 deltaNCD

Plasmid

#124920

Purpose

For expression of N-terminal Conserved Domain deletion mutant of SIN1-GFP (delta2-137)

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-Elf5 gRNA

Plasmid

#128836

Purpose

gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

mElf5 gRNA (Elf5 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Klf5

Plasmid

#128830

Purpose

dox-dependent expression of mouse Klf5

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Klf5 (Klf5 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLD1

Plasmid

#160805

Purpose

Express POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLD1 (POLD1 Human)

Use

Retroviral

Tags

Expression

Mutation

Codon optimized C-terminal 230 amino acids

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-mβTrCP WD1-7 (241-569)

Plasmid

#62977

Purpose

Expresses myc-tagged mouse βTrCP residues 241-569

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

codes for residues 241-569

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

Plasmid

#128835

Purpose

Knock-in of TagBFP into mouse Esrrb locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

tagBFP into Esrrb locus (Esrrb Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Xpress beta4 1355T

Plasmid

#16040

Depositor

Filippo Giancotti

Article

Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

Insert

beta4 (ITGB4 Human)

Use

Tags

6xHis and Xpress

Expression

Mammalian

Mutation

1355T: amino acids 1-1355

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 myc-mβTrCP F-box (1-247)

Plasmid

#62976

Purpose

Expresses myc-tagged mouse βTrCP residues 1-247

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Use

Tags

3x myc

Expression

Mammalian

Mutation

codes for residues 1-247

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pFUGW-shRIIα-RIIαWT-IRES-EGFP

Plasmid

#183456

Purpose

Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Tags

Expression

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM45_WT

Plasmid

#82892

Purpose

Gateway Donor vector containing RBM45, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM45 (RBM45 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-Esrrb gRNA

Plasmid

#128841

Purpose

gRNA for targeting mouse Esrrb locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Esrrb gRNA (Esrrb Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/E2F2/NRF-1(-64)-like

Plasmid

#66742

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCG(-64) to TACA

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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161+80pCalb2/mutCREB(-2)-like

Plasmid

#66751

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp +80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

GCGC (-2) to TAGA

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/mutNRF-1(-41)-like

Plasmid

#66744

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGC (-41) to ATT

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/dmutNRF-1(-41;-35)-like

Plasmid

#66741

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCAGGCGC (-41;-35) to ATTAGGATT

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

Plasmid

#62978

Purpose

Expresses myc-tagged mouse βTrCP residues 292-569

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

codes for residues 292-569

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pGMC00035 (aka pMG0784)

Plasmid

#210768

Purpose

Lentiviral vector to express human ADRM1

Depositor

Raj Chari

Article

Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

Insert

ADRM1 (ADRM1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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