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pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90288

Purpose

BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE63

Plasmid

#79445

Purpose

recipient plasmid [single nuclease]; p35S:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE144

Plasmid

#79448

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE148

Plasmid

#79450

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE151

Plasmid

#79451

Purpose

recipient plasmid [multiplexing]; pICU2:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE160

Plasmid

#79452

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE164

Plasmid

#79454

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE165

Plasmid

#79455

Purpose

recipient plasmid [multiplexing]; pICU2:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE166

Plasmid

#79456

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE169

Plasmid

#79459

Purpose

recipient plasmid [multiplexing]; pICU2:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE2

Plasmid

#79441

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJK_proNR2_LGK.9

Plasmid

#81162

Purpose

Expresses levoglucosan kinase variant LGK.9 with promoter proNR2

Depositor

Timothy Whitehead

Article

Klesmith et al ACS Synth Biol. 2015 Sep 14.

Insert

Levoglucosan kinase

Tags

His6x

Expression

Bacterial

Mutation

LGK.9 - (T15R, A57Q, R94G, R100C, N101G, S105R, L…

Promoter

proNR2

Available Since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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ARK2a

Plasmid

#81017

Purpose

E.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Inserts

MevTo-BBa1002-pTrc-Mkco
PMD

Promoter

LacUV5 and Trc promoter

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pJL1702

Plasmid

#78195

Purpose

optogenetic activation of IL2 neuron in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

ChR2(gf)

Expression

Worm

Promoter

lag-2

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pJL1603

Plasmid

#78193

Purpose

Ce cha-1 expression in SMB, SIA neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

odr-2

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL580

Plasmid

#78185

Purpose

Ce rpy-1 expression in worms

Depositor

Junho Lee

Article

Nam et al J Biol Chem. 2009 Mar 20;284(12):8195-20

Insert

rpy-1 (rpy-1 Nematode)

Expression

Worm

Promoter

rpy-1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL1602

Plasmid

#78192

Purpose

Ce cha-1 expression in SIA, RMD neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

trp-1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL1601

Plasmid

#78191

Purpose

Ce cha-1 expression in IL2, SIA neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

osm-6

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-dSestrin (CG11299-PD)

Plasmid

#73675

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn (variant D) (Sesn Fly)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pDS2-aurZ

Plasmid

#70151

Purpose

Fusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

aurZ

Expression

Yeast

Promoter

Tef

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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