We narrowed to 13,568 results for: cas9 genes

1. pET302-6His-dCas9-Halo

   Plasmid

   #72269

   Purpose

   Encodes a Halo-labeled fusion of nuclease deficient Cas9

   Depositor

   Timothée Lionnet

   Article

   Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

   Insert

   His6-dCas9-Halo
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZR071_SFFV-dCas9-mCherry-KRAB

   Plasmid

   #180264

   Purpose

   Lentiviral expression vector for dCas9-KRAB in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   KRAB and mCherry

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-Cas9-gRNA-TagBFP2

   Plasmid

   #124774

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pb-TRE3G-SpCas9-IRES-blast

   Plasmid

   #195506

   Purpose

   Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-MMLVgag-3xNES-Cas9

   Plasmid

   #181752

   Purpose

   MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLV-Gag-3xNES-Cas9 (Spy)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRT043-CLYBL-DDdcas9-VPH

   Plasmid

   #158091

   Purpose

   Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

   Depositor

   Martin Kampmann

   Article

   Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

   Insert

   CLYBL-CAG-DDdCas9VPH-T2A-GFP
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-SaCas9-U6-sgRNA

   Plasmid

   #124844

   Purpose

   Vector for Cre-dependent expression of SaCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SaCas9, gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS and NLS-3xHA

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-dCas9-VP64-6xHis

   Plasmid

   #62935

   Purpose

   Expression of dCas9-VP64-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   dCas9-VP64
   

   Use

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A and H840A amino acid changes render Cas9 nuc…

   Promoter

   T7

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-Cas9-gRNA-GFP

    Plasmid

    #124770

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC13N-dCas9-BFP-KRAB

    Plasmid

    #127968

    Purpose

    constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-dCas9-NLS-BFP-KRAB
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3TS-spCas9 ABE-Umax

    Plasmid

    #222138

    Purpose

    Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9 (cas9 Synthetic)
    

    Use

    CRISPR; Zebrafish expression

    Tags

    bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-28b-Cas9-His

    Plasmid

    #47327

    Purpose

    For in vitro expression and purification of Cas9 protein

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEvolvR-enCas9-PolI3M-TBD

    Plasmid

    #113077

    Purpose

    Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber, David Schaffer

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI3M-TBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. P3-dCas9-Tet1-GFP-Puro

    Plasmid

    #190728

    Purpose

    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    3XFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    pCAG

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-NLS-Cas9-6xHis

    Plasmid

    #62934

    Purpose

    Expression of NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    NLS-Cas9-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX303-ZIM3-KRAB-dCas9

    Plasmid

    #154472

    Purpose

    Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xFLAG-dCas9/pCMV-7.1

    Plasmid

    #47948

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A (catalyticall…

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpCas9 nickase for Split-PE (pJUL2497)

    Plasmid

    #190104

    Purpose

    Expresses nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    Tags

    XTEN-bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti_EFS-Cas9-P2A-HygR

    Plasmid

    #164134

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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