We narrowed to 40,066 results for: kan

1. pKEE401

   Plasmid

   #91715

   Purpose

   CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRelA'

   Plasmid

   #175595

   Purpose

   Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Catalytic domain of E.coli relA gene.
   

   Expression

   Bacterial

   Mutation

   Only the catalytic domain of the enzyme is used (…

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSmart-EF1a-Nla-cas5

   Plasmid

   #178878

   Purpose

   Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas5c-bGH polyA
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSmart-EF1a-Nla-cas8

   Plasmid

   #178880

   Purpose

   Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas8c-bGH PolyA
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSmart-EF1a-Nla-cas11

   Plasmid

   #178882

   Purpose

   Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas11c-bGH polyA
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSmart-EF1a-Nla-cas7

   Plasmid

   #178879

   Purpose

   Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas7c-bGH PolyA
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBC155

   Plasmid

   #202274

   Purpose

   Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker

   Depositor

   Paul Schulze-Lefert

   Article

   Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

   Insert

   bc182
   

   Expression

   Bacterial

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBC114

   Plasmid

   #202276

   Purpose

   Level 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

   Depositor

   Paul Schulze-Lefert

   Article

   Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

   Insert

   bc141
   

   Expression

   Bacterial

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHC01

   Plasmid

   #106900

   Purpose

   Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

   Depositor

   Jonathan Silberg

   Article

   Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

   Inserts

   LasR
   Methyl Halide Transferase
   Ethylene Forming Enzyme
   Red Fluorescent Protein
   

   Expression

   Bacterial

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH67131

    Plasmid

    #153223

    Purpose

    Level 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminator

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Nos promoter, NPTII, Nos terminator
    

    Use

    Synthetic Biology

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pconst #5-RFP

    Plasmid

    #81079

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23110

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pconst #4-RFP

    Plasmid

    #81078

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23107

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pconst #1-RFP

    Plasmid

    #81075

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23119

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCALNL-eGFP-NT1-6-gix-6-NT1

    Plasmid

    #81204

    Purpose

    contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Expression

    Mammalian

    Promoter

    pCBa

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-APEX2-mCherry

    Plasmid

    #188944

    Purpose

    Middle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry

    Depositor

    Owen Tamplin

    Article

    Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

    Insert

    mito-APEX2_p2A_APEX2-H2B_p2A_mCherry
    

    Use

    Middle entry vector for gateway (l1-l2)

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pconst #3-RFP

    Plasmid

    #81077

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23101

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pconst #2-RFP

    Plasmid

    #81076

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter- BBa_J23104

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBER

    Plasmid

    #62662

    Purpose

    Donor vector for tdTomato knock-in via landing pad

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Inserts

    tdTomato
    hygromycin
    

    Use

    Cre/Lox

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBGX

    Plasmid

    #62666

    Purpose

    Donor vector for Dre knock-in via landing pad

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    Dre
    

    Use

    Cre/Lox

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBGO

    Plasmid

    #62671

    Purpose

    Donor vector for mFTH1 knock-in via landing pad

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    FTH1
    

    Use

    Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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