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166,787 results

Showing: 1641 - 1660 of 166787 results

  1. pCMV-PE6c

    Plasmid
    #207853
    Purpose
    Mammalian expression of PE6c prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6c
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207853-plasmid-map-sequence-id-423164

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  3. PRESTO-Tango Kit

    Plasmid Kit
    #1000000068
    Purpose
    Used to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.
    Depositor
    Bryan Roth
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5)

    Viral Prep
    #52925-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pZac2.1 gfaABC1D-cyto-GCaMP6f (#52925). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-cyto-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) cyto-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Article
    Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1
    Promoter
    Tags
    None
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. pET21a-Streptavidin-Alive

    Plasmid
    #20860
    Purpose
    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Alive
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Wt gene
    Promoter
    Available Since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20860-plasmid-map-sequence-id-194024

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  10. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available Since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    89308-plasmid-map-sequence-id-425605

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  11. pAAV-hSyn-mCherry (AAV8)

    Viral Prep
    #114472-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only

  12. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106280-plasmid-map-sequence-id-354474

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  13. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV Retrograde)

    Viral Prep
    #44362-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available Since
    June 21, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. pAAV-hSyn-Cre-P2A-dTomato (AAV PHP.eB)

    Viral Prep
    #107738-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Rylan Larsen
    Promoter
    Syn
    Tags
    dTomato (physically separate, not a fusion protein)
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3.1-HA

    Plasmid
    #128034
    Purpose
    Mammalian expression vector with N terminal HA tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128034-plasmid-map-sequence-id-422971

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  16. pGP-AAV-syn-iGABASnFR2-WPRE

    Plasmid
    #218874
    Purpose
    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)
    Depositor
    GENIE Project
    Article
    Kolb et al bioRxiv 2025.03.25.644953
    Has Service
    AAV1 and AAV5
    Insert
    iGABASnFR2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S99A F102Y F104Y L178S
    Promoter
    Synapsin
    Available Since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218874-plasmid-map-sequence-id-436585

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  17. pAAV-hSyn-hM3D(Gq)-mCherry (AAV9)

    Viral Prep
    #50474-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-hSyn-GRAB-gDA3m (AAV1)

    Viral Prep
    #208698-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-GRAB-gDA3m (#208698). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB-gDA3m plasmid DNA. Syn-driven expression of the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Promoter
    Syn
    Tags
    None
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV-CAG-GFP (AAV5)

    Viral Prep
    #37825-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available Since
    Oct. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 1641 - 1660 of 166787 results