182,051 results
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Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase
UseLuciferaseExpressionMammalianMutationdestabilizing sequences (DS) added to the C termi…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FH-Tet2-pEF
Plasmid#41710PurposeThis plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian systemDepositorInserttet methylcytosine dioxygenase 2 (Tet2 Mouse)
TagsFLAG and HAExpressionMammalianPromoterEF1aAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-MCS2
Plasmid#46954PurposeCloning plasmid for the generation of Knock-In in human cells using rAAV.DepositorPublicationTypeEmpty backboneUseAAVAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-R-GECO1.2
Plasmid#45494PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertR-GECO1.2
ExpressionMammalianMutationR-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR1A-Huwe1
Plasmid#37431DepositorInsertHuwe1 (HUWE1 Human)
UseGateway CloningAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CCSB-Broad Human Kinase ORF Collection
Plasmid kit#1000000014PurposePlasmids for over 500 human kinases and kinase-related proteins in Gateway entry vectors.DepositorApplicationGene Expression and LabelingVector typeMammalian ExpressionAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
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p6XHis-T7(P266L)
Plasmid#174866PurposeExpresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.DepositorInsertT7 RNA polymerase
Tags6XHis-tagExpressionBacterialMutationP266LPromoterlac promoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV PHP.eB)
Viral prep#44362-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with the PHP.eB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
ExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-CD9-10
Plasmid#55013PurposeLocalization: Tetraspanin/Membrane, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD9 (CD9 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pANAP
Plasmid#48696PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
ExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-neo-mOVA
Plasmid#25099DepositorInsertmembrane bound ovalbumin
ExpressionBacterial and MammalianAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-myc3-CUL4B
Plasmid#19922DepositorPublicationAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-CD14
Plasmid#13645DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD14 (CD14 Human)
ExpressionMammalianAvailable sinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB
Plasmid#154473PurposeExpresses dCas9 fused to mCherry and the KRAB domain of ZIM3DepositorInsertZIM3 (ZIM3 Human)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterSFFVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-MBP-mSA2
Plasmid#52319PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
TagsFLAG, His, MBP, and TEVExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
Plasmid#46168PurposeCodon optimized Cas9_SV40 NLS with intronDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcodon optimized Cas9_SV40 NLS with intron
UseCRISPRMutationintron inserted at position 3113-3163, and SV40 N…Promotereef-1A.1 (eft-3)Available sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-mSmo
Plasmid#25395DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmouse Smoothened cDNA (Smo Mouse)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pROSA26-PA
Plasmid#21271DepositorInsertROSA26 (Gt(ROSA)26Sor Mouse)
UseMouse TargetingAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by