161,697 results

1. pMSP3535

   Plasmid

   #46886

   Purpose

   Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

   Depositor

   Gary Dunny

   Article

   Bryan et al Plasmid. 2000 Sep;44(2):183-90.

   Type

   Empty backbone

   Use

   Nisin-inducible expression; gram-positive bacteri…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PnisA

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Autobow

   Plasmid

   #45182

   Depositor

   Joshua Sanes

   Article

   Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

   Insert

   pCAG-Autobow
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. APOBEC3G_pLX307

   Plasmid

   #98345

   Purpose

   Lentiviral expression of APOBEC3G

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   APOBEC3G (APOBEC3G Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. HaloTag-beta2-adrenergic receptor

   Plasmid

   #66994

   Purpose

   Beta-2-adrenergic receptor tagged with HaloTag on amino terminus

   Depositor

   Catherine Berlot

   Insert

   HaloTag-beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   Human Growth hormone Signal Sequence-HaloTag

   Expression

   Mammalian

   Mutation

   The signal sequence from human growth hormone was…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ai65(RCFL-tdT) targeting vector

   Plasmid

   #61577

   Purpose

   Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   pCAG-FSF-LSL-tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-hERG1a

   Plasmid

   #53052

   Purpose

   Expresses alpha subunit of hERG1a K channel in mammalian cells

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLViP-LifeAct-iRFP670

   Plasmid

   #229699

   Purpose

   Lentiviral expression of LifeAct-iRFP670 in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   Synthetic construct LifeAct-mTurquoise gene
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-Ftractin-mCherry

   Plasmid

   #85131

   Purpose

   lentiviral expression of an F-actin live-cell marker (mCherry)

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-dLight1.3b (AAV9)

   Viral Prep

   #125560-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Promoter

   CAG

   Tags

   None

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

10. NTHL1-mCherry

    Plasmid

    #215145

    Purpose

    Expresses NTHL1-mCherry in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    NTHL1 (NTHL1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-TORCAR(T/A)

    Plasmid

    #64928

    Purpose

    Negative-control mutant for TORCAR biosensor.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR(T/A) (EIF4EBP1 Synthetic, Human)
    

    Use

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Mutation

    4EBP1 domain contains T37A and T46A mutations.

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-Myc-Caspase-5

    Plasmid

    #214305

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP5 (CASP5 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEVOL-pAzF

    Plasmid

    #31186

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

    Depositor

    Peter Schultz

    Article

    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

    Insert

    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32T, E107N, D158P, I159L, L162Q, D286R

    Promoter

    Available since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

    Plasmid

    #159697

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX-EF1a_NKX2-1_Long

    Plasmid

    #221483

    Purpose

    NKX2-1 long isoform lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9 (PX165)

    Plasmid

    #48137

    Purpose

    Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV MP-84 mRFP

    Plasmid

    #206926

    Purpose

    The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.

    Depositor

    Markus Grompe

    Article

    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

    Insert

    mRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MP-84

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMBP-hLIF

    Plasmid

    #179711

    Purpose

    Expresses H6-MBP-LIF

    Depositor

    Mark Arbing

    Insert

    Leukemia inhibitory factor (LIF Human)
    

    Use

    Tags

    His6-MBP-TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 35S-eGFP-nosT

    Plasmid

    #80127

    Purpose

    Transient expression vector for eGFP in plants

    Depositor

    Yutaka Kodama

    Article

    Fujii et al Plant Biotech. 2015; 32(1): 81-87.

    Type

    Empty backbone

    Use

    Tags

    eGFP

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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