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We narrowed to 18,824 results for: nls

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Showing: 1641 - 1660 of 18824 results

  3. pCMVMagFRET-1-NLS

    Plasmid
    #50748
    Purpose
    Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.
    Depositor
    Maarten Merkx
    Publication
    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gene encoding for MagFRET-1 with NLS
    Expression
    Mammalian
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50748-plasmid-map-sequence-id-76084

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  5. CMVp-dCas9-3xNLS-VP64 (Construct 1)

    Plasmid
    #55195
    Purpose
    Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.
    Depositor
    Timothy Lu
    Publication
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    VP64
    Expression
    Mammalian
    Promoter
    CMV/hUBC
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55195-plasmid-map-sequence-id-85582

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  7. xCas9(3.6)-ABE(7.10)

    Plasmid
    #108387
    Purpose
    Mammalian expression vector for xCas9(3.6)-ABE
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.6)-ABE(7.10)
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108387-plasmid-map-sequence-id-207183

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  8. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104907-plasmid-map-sequence-id-206042

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  14. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  16. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

    Plasmid
    #123616
    Purpose
    CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-nCas9-NLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A in SpCas9
    Promoter
    CMV
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123616-plasmid-map-sequence-id-239344

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Showing: 1641 - 1660 of 18824 results