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pFLinkC-AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV

Plasmid

#137118

Purpose

Protease Switch Clone ID: 2-B3

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV

Use

Synthetic Biology

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-eYFP (AAV9)

Viral Prep

#117382-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

Syn

Tags

eYFP

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only
IDP424

Plasmid

#87018

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

CLIP-Nrx1B-1xλN(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pNTI825

Plasmid

#216633

Purpose

Cal-ID (V5) for AAV production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID

Use

AAV

Tags

V5

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRC241

Plasmid

#225981

Purpose

J23117_RC2_mTagBFP_Buj_mRFP dual operon reporter

Depositor

Jesse Zalatan

Article

Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

Insert

mTagBFP (with rc RBS), mRFP (with buj RBS)

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7FTrp)RS

Plasmid

#177310

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-eYFP (AAV Retrograde)

Viral Prep

#117382-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

Syn

Tags

eYFP

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only
pEN_TGmiR_Luc-A

Plasmid

#25765

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Gateway Cloning

Tags

GFP

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-Luc

Plasmid

#25739

Purpose

Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSLIK-Neo-TmiR-Luc

Plasmid

#25743

Purpose

Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pBXPHM3-PCFT_NB

Plasmid

#165415

Purpose

Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT_NB (SLC46A1 Chicken, Llama)

Tags

His10-MBP-3C protease site

Expression

Bacterial

Promoter

arabinose

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pet151D-TOPO BROX 1-411;

Plasmid

#89602

Purpose

Expresses full length human Brox in bacteria; internal ID WISP11-297

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

BROX (BROX Human)

Tags

His Tag, V5 tag

Expression

Bacterial

Promoter

T7

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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jk64

Plasmid

#87012

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

Nrx1B-4xλN(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4C (216-233) (pGEX2T(TEV))

Plasmid

#80635

Purpose

Residues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4C (CHMP4C Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 216-233

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4A (1-204) (pGEX2T(TEV))

Plasmid

#80632

Purpose

Residues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-204

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4B (205-224) (pGEX2T(TEV))

Plasmid

#80634

Purpose

Residues 205-224 of CHMP4B, Bacterial Expression Vector; TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4B (CHMP4B Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 205-224

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-2H12

Plasmid

#221186

Purpose

His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p2H12

Plasmid

#221142

Purpose

cdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen

Expression

Bacterial

Promoter

Ptet

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCINeoFlag ALIX; I318A

Plasmid

#89867

Purpose

Expresses full length Flag-tagged ALIX with mutation I318A in mammalian cells; internal ID WISP11-403

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

ALIX (PDCD6IP Human)

Tags

Flag

Expression

Mammalian

Mutation

Mutated isoleucine 318 to alanine

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-RBP2

Plasmid

#241990

Purpose

Mouse RBP2 (accession ID NP_001074857.1), CMV promoter-enhancer, downstream WPRE.

Depositor

Tobias Moser

Article

Kapoor et al Elife. 2026 Jan 13;13:RP98254. doi: 10.7

Insert

RIM-BP2 (Rimbp2 Mouse)

Use

AAV

Tags

RIM-BP2 (untagged)

Expression

Mammalian

Promoter

CMV enhancer

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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