We narrowed to 2,202 results for: K-Ras protein

1. RNA motif plasmid cloning backbone

   Plasmid

   #107253

   Purpose

   RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

   Depositor

   Paul Khavari

   Article

   Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

   Insert

   RNA motif plasmid cloning backbone
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2 Krt4 Src (YF)-uniRapR-cerulean

   Plasmid

   #45509

   Depositor

   Klaus Hahn, Anna Huttenlocher

   Article

   Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   Src (YF)-uniRapR-cerulean
   

   Use

   Zebrafish expression

   Tags

   cerulean

   Expression

   Mutation

   Y732F

   Promoter

   krt4

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. NPY-td-Orange2

   Plasmid

   #83497

   Purpose

   Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

   Depositor

   Sebastian Barg

   Article

   Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

   Insert

   NPY (NPY Human)
   

   Use

   Tags

   tandem mOrange2

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

   Plasmid

   #156463

   Purpose

   Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

   Depositor

   Andre Stiel

   Article

   Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

   Inserts

   Heme Oxygenase
   ReBphP-PCM
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP

   Plasmid

   #114106

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al J Neurosci Res. 2019 Apr 7. doi: 10.1002

   Insert

   Gaussia luciferase, slow burn; Channelrhodopsin 2(CS/DA), stable step function opsin; EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTH848-gst-disec

   Plasmid

   #89589

   Purpose

   Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase codon-dis-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 3xFLAG TPP1 88-544 delta166-172

   Plasmid

   #53551

   Purpose

   mammalian expression of TPP1 full length with 166-172 replaced with "GSSG"

   Depositor

   Kathleen Collins

   Article

   Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

   Insert

   TPP1 (ACD Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   contains aa 88-544, with aa 166-172 replaced wit…

   Promoter

   CMV

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1 3xFLAG TPP1 88-249 delta166-172

   Plasmid

   #53550

   Purpose

   mammalian expression of TPP1 OB-fold domain with 166-172 replaced with "GSSG"

   Depositor

   Kathleen Collins

   Article

   Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

   Insert

   TPP1 (ACD Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   contains aa 88-249, with aa 166-172 replaced with…

   Promoter

   CMV

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pQCXIX_sh_HOIP/RNF31_#6

   Plasmid

   #75252

   Purpose

   retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   shRNA against human RNF31 (RNF31 )
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-AVITEV-GFP

    Plasmid

    #74052

    Purpose

    retroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    AVITEV-GFP
    

    Use

    Retroviral

    Tags

    AVI-TEV-tag N-terminal to the GFP

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hRREB1-RFP

    Plasmid

    #22924

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    human ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDest-303

    Plasmid

    #159678

    Purpose

    Gateway destination vector for mammalian protein expression

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME18S-3HA-mULK2(K39T)

    Plasmid

    #22899

    Depositor

    Masaaki Muramatsu

    Article

    Yan et al Oncogene. 1999 Oct 21. 18(43):5850-9.

    Insert

    UNC-51-like kinase 2 (Ulk2 Mouse)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed Lysine 39 to Threonine

    Promoter

    Available since

    Sept. 14, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pAAVS1-P-CAG-GFPluc2

    Plasmid

    #80493

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    GFPluc2
    

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-sbGLuc-CheRiff-EGFP

    Plasmid

    #114108

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.

    Insert

    Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pF CAG luc-EGFP-cre puro

    Plasmid

    #67503

    Purpose

    Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    luciferase-EGFP-cre
    

    Use

    Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCellFree_G03 POLD1

    Plasmid

    #67128

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCellFree_G03 RRN3

    Plasmid

    #67112

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RRN3 (RRN3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pTH847-gst-optsc

    Plasmid

    #96908

    Purpose

    Plasmid for yeast expression of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    yeast codon optimised glutathione-S transferase
    

    Use

    Tags

    8xhistidine

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTH861-gst-optec P210T

    Plasmid

    #89595

    Purpose

    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed proline 210 to threonine

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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