Purpose
hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
Inserts
HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
Available Since
April 8, 2019
Availability
Academic Institutions and Nonprofits only