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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,444 results for: grn

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Showing: 1661 - 1680 of 15444 results

  4. pX330 EGFR-sgRNA

    Plasmid
    #188633
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR
    Depositor
    Jared Toettcher
    Publication
    Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    188633-plasmid-map-sequence-id-369478

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  7. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  9. pX330_TUBA1B sgRNA / hSpCas9

    Plasmid
    #172834
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172834-plasmid-map-sequence-id-340790

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  10. Plas-gRNA-Z3EV

    Plasmid
    #157659
    Purpose
    GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.
    Depositor
    Frank Albert
    Publication
    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP-gRNA NatMX Z3EV
    Use
    Insert storage (replicative in e. coli)
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    157659-plasmid-map-sequence-id-309102

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Showing: 1661 - 1680 of 15444 results