We narrowed to 4,624 results for: T2A

1. pET-His6-Sumo-IntNtr-eSrt2A

   Plasmid

   #126015

   Purpose

   Bacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)

   Depositor

   Thomas Muir

   Insert

   His6-Sumo-IntNtr-eSrt2A
   

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2ADW-lox-mCherryNLS-pA-lox-4493NES

   Plasmid

   #138374

   Purpose

   Booster-PKA for the generation of mice

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   lox-mCherry-lox-Booster-PKA
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMT-PT2A-Tdtomato-iRFP670-GFP

   Plasmid

   #111815

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   Tdtomato
   iRFP670
   GFP
   

   Use

   Cloning intermediate

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

   Plasmid

   #129028

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-Cas9-T2A-STOP-TdT

   Plasmid

   #126425

   Purpose

   Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)
   

   Expression

   Mammalian

   Mutation

   alanine-6 of TdT has been changed to a STOP codon

   Promoter

   pCMV

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-PHR-iSH-T2A-CIBNcaax

   Plasmid

   #173860

   Purpose

   Mammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K)

   Depositor

   Takao Nakata

   Article

   Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

   Insert

   mCherry-PHR-iSH-T2A-CIBNcaax
   

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

   Plasmid

   #172296

   Purpose

   In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing

   Depositor

   Patrik Verstreken

   Insert

   Cas9n-RT-T2A-PuroR
   

   Use

   CRISPR

   Mutation

   H840A (Cas9)

   Promoter

   T7 promoter A-initiating

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70

   Plasmid

   #62944

   Purpose

   Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-QF2-Hsp70
   

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

   Plasmid

   #62946

   Purpose

   Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-QF2-Hsp70
   

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4

    Plasmid

    #193348

    Purpose

    tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2-17-t2a-Klf4 (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    TetO mini CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV hSyn1 CaRhAC T2A tDimer

    Plasmid

    #101722

    Purpose

    - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

    Depositor

    Thomas Oertner

    Article

    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

    Inserts

    CatRhAC
    red fluorescent protein
    

    Use

    AAV

    Mutation

    E497K,C569D

    Promoter

    human Synapsin1 promotor and ribosomal skip seque…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-EFS-hTSC1-T2A-Bsd

    Plasmid

    #193199

    Purpose

    Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    TSC1 (TSC1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-miniSOG-T2A-dTomato

    Plasmid

    #193796

    Purpose

    Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-hSyn-IntCCCre-T2A-CopGFP

    Plasmid

    #160503

    Purpose

    Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCXLE-dCas9VPP300-T2A-GFP-shP53

    Plasmid

    #102896

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPP300-T2A-GFP-shP53
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRE-UBC-Crry-T2A-GFP

    Plasmid

    #205452

    Purpose

    lentiviral plasmid for expression of mouse Crry

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    Crry (Cr1l Mouse)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dppa1-T2A-H2B-Halo donor

    Plasmid

    #113118

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Dppa1 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid

    #172298

    Purpose

    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes

    Depositor

    Patrik Verstreken

    Insert

    CRE-T2A-PuroR
    

    Use

    Cre/Lox

    Promoter

    T7 promoter A-initiating

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-EF1a-tetR-T2A-tSapphire

    Plasmid

    #176312

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    T-sapphire
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Gr4-T2A-QF2 HDR plasmid

    Plasmid

    #140944

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene

    Depositor

    Leslie Vosshall

    Article

    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

    Insert

    Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
    

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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