164,909 results

1. pET21a-ProRS-His

   Plasmid

   #124117

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Prolyl-tRNA ligase (proS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. His 3C MKK6 1-334

   Plasmid

   #87881

   Purpose

   E. coli expression of MKK6 aa 1-334

   Depositor

   Peter Mace

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   3C protease site and 6xHis

   Expression

   Bacterial

   Mutation

   aa 1-334

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-PASS

   Plasmid

   #193970

   Purpose

   Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

   Depositor

   Guangwei Du

   Article

   Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

   Insert

   PA biosenor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-Rab11a-7

   Plasmid

   #55124

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab11a (RAB11A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFD6

   Plasmid

   #73915

   Purpose

   tissue-specific multiplex gRNA plasmid

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   :tRNA:gRNA:tRNA:gRNA:tRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAS-DSCP

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-nCas9n

   Plasmid

   #47929

   Purpose

   expression of an optimized Cas9 for genome-editing in zebrafish

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   nls-zcas9-nls
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-GFP

   Plasmid

   #86537

   Purpose

   Retrovirus expressing GFP

   Depositor

   Xue Han

   Article

   Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

   Insert

   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin I (human)

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. plex_306-ccdB-g418

   Plasmid

   #201133

   Purpose

   pLEX_306 plasmid with g418 marker

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. N370S GBA pcDNA3.1

   Plasmid

   #188583

   Purpose

   Ectopic expression N370S GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N370S

   Promoter

   CMV

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid

    #85756

    Purpose

    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Inserts

    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    

    Use

    CRISPR

    Tags

    Csy4, Csy4 recognition sequence, FokI fusion via …

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CMV and U6

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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