161,271 results

1. pLVX-EGFP-IRES-puro

   Plasmid

   #128652

   Purpose

   mammalian expression of EGFP with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   CMV

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-hygro CDK4 R24C

   Plasmid

   #11254

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   R24C

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-Rheb (Q64L)-pcw107

   Plasmid

   #64607

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   RHEB (RHEB Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Q64L

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

4. p-cyclinB1-mcherry (1958)

   Plasmid

   #26063

   Depositor

   Jonathon Pines

   Article

   Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-OSF-PP

   Plasmid

   #154247

   Purpose

   backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Type

   Empty backbone

   Use

   Tags

   FLAG and Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3XFLAG-VP64-SadCas9-NLS-VP64

   Plasmid

   #135338

   Purpose

   3xFLAG 2xVP64 SadCas9 for single guide cloning

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   SadCas9
   

   Use

   Tags

   3xFLAG-VP64-SV40 NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_SynP_DIO_tAKARα _WPRE

   Plasmid

   #196675

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   tAKARα
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-SCN1B-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156534

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SCN1B (SCN1B Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-hXRCC1

   Plasmid

   #70759

   Purpose

   bacterial expression of human XRCC1

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   XRCC1 (XRCC1 Human)
   

   Use

   Tags

   6-HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lac operon

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MLM3613

    Plasmid

    #42251

    Purpose

    Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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