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1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA
Plasmid#109318PurposePlasmid for liver-specific expression of EmGFP with a gRNA against SaCas9DepositorInsertSaCas9 RuvC gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA
Plasmid#108848PurposeInsert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagspMag,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
NKP68 RFP (790)
Plasmid#62003Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNKP68 (TMUB1 Human)
UseTagsmRFPExpressionMammalianMutationPromoterpgkAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)-SPN+IFS
Plasmid#83372PurposeExpresses SPN and IFS complex in E. coli cellDepositorInsertsSPN
IFS
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pBS NL4-3 envFS eGFP 5'CMV Δtat
Plasmid#101347Purposetat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.DepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta TatPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV FLIP p53
Plasmid#19745DepositorInsertoligo targeting p53 (Trp53 Mouse)
UseCre/Lox, RNAi, and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA
Plasmid#108846PurposeInsert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsnMag,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 EBNA1BP2
Plasmid#67072PurposeStandard for cell-free expression benchmarking.DepositorInsertEBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo-TmiR-Luc
Plasmid#25743PurposeControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.DepositorInsertLuciferase miR-shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2E1
Plasmid#67089PurposeStandard for cell-free expression benchmarking.DepositorInsertGTF2E1 (GTF2E1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE60-mPU.1-ETS
Plasmid#85733PurposeExpresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4DepositorInsertETS domain of transcription factor PU.1 (Spi1 Mouse)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA
Plasmid#108845PurposeInsert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGAIExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA
Plasmid#108843PurposeInsert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1ExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP3_Z
Plasmid#148151PurposeMammalian Expression of Hs4EBP3DepositorInsertHs4EBP3 (EIF4EBP3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s-T78H
Plasmid#100023PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutationDepositorInsertsfGCaMP6s-T78H
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 FOXM
Plasmid#67118PurposeStandard for cell-free expression benchmarking.DepositorInsertFOXM (FOXM1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
DsRed +9e
Plasmid#191766PurposeExpression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9eDepositorInsertDsRed
UseTagsN terminal fusion of positively charged polypetod…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWKS12
Plasmid#139419PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker.DepositorInsertSuperfolder GFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only
