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We narrowed to 4,360 results for: PORA

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Showing: 1701 - 1720 of 4360 results

  2. pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

    Plasmid
    #176087
    Purpose
    EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Lys72 to Ala, and mutation in the PAM…
    Promoter
    CMV
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    176087-plasmid-map-sequence-id-352865

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  4. pUC19-POLBHR-eGFP

    Plasmid
    #176064
    Purpose
    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    EGFP-PolB (POLB Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    pUC with endogenous SapI mutated out, PAM mutatio…
    Promoter
    N/A
    Available Since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176064-plasmid-map-sequence-id-352734

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  5. pUltraI-Tet3.0[TAA]

    Plasmid
    #164580
    Purpose
    Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mb Tet3.0RS (R2-3)
    Mm tRNA[TAA]
    Expression
    Bacterial
    Mutation
    L170G, N311G, C313A
    Promoter
    ProK and TacI
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    164580-plasmid-map-sequence-id-392917

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  10. pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500

    Plasmid
    #92315
    Purpose
    Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    B1 domain of protein L (PL) mutant K16TAG
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    p70b (p70a promoter with one mutation)
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    92315-plasmid-map-sequence-id-197049

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  11. pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500

    Plasmid
    #92319
    Purpose
    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    p70a
    Available Since
    July 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    92319-plasmid-map-sequence-id-182040

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  12. pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500

    Plasmid
    #92318
    Purpose
    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    p70a
    Available Since
    July 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    92318-plasmid-map-sequence-id-182037

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  13. pEVOL-pAzF[TAG]

    Plasmid
    #164579
    Purpose
    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    araBAD, glnS, and proK
    Available Since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    164579-plasmid-map-sequence-id-392918

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  14. pSLIK_hygro_3xflag_NRF2(RR)

    Plasmid
    #136535
    Purpose
    Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)
    Depositor
    Kevin Janes
    Article
    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13
    Insert
    NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    This plasmid is developed by mutating 3 base pair…
    Available Since
    July 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    136535-plasmid-map-sequence-id-304676

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  17. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)

    Plasmid
    #176144
    Purpose
    EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Gly60 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176144-plasmid-map-sequence-id-353152

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  18. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)

    Plasmid
    #176143
    Purpose
    EGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Arg65 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176143-plasmid-map-sequence-id-353151

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  19. pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro

    Plasmid
    #176089
    Purpose
    EGFP fused the N-terminus of POLB containing the mutations Lys35Ala, Lys68Ala, and Lys72Ala, a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutations in Lys35 to Ala, Lys68 to Ala, and Lys7…
    Promoter
    CMV
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176089-plasmid-map-sequence-id-352910

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Showing: 1701 - 1720 of 4360 results