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Myc-E-Syt2

Plasmid

#66834

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Publication

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt2 (ESYT2 Human)

Tags

Myc

Expression

Mammalian

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pWZL Neo Myr Flag LCK

Plasmid

#20511

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

LCK (LCK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
[#GS7-LV] DsRed GFP TEVp Zeo Switch - Recipient

Plasmid

#233507

Purpose

Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

Depositor

Simon Grelet

Publication

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

DsRed loxP eGFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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[#GS16-LV] Syn1-MitoTRACER - Donor

Plasmid

#233565

Purpose

Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Publication

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Lentiviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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HcKCR1_mCherry_pcDNA3.1

Plasmid

#177336

Purpose

Expresses KCR1 in mammalian cells

Depositor

John Spudich

Publication

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR1

Tags

mCherry

Expression

Mammalian

Mutation

mammalian codon-adapted

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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METTL3 shRNA 1 tet pLKO puro

Plasmid

#162985

Purpose

Tet-inducible shRNA targeting human METTL3 #1

Depositor

Mark Rubin

Publication

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO5d.EFS.SpCas9.P2A.PAC

Plasmid

#58329

Purpose

Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA3.1(+)-NEMOc

Plasmid

#189933

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Publication

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOc

Expression

Mammalian

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166017

Purpose

mammalian expression of HCoV-NL63 soluble spike trimer protein

Depositor

Dominic Esposito

Publication

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-NL63 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWZL Neo Myr Flag GRK5

Plasmid

#20495

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

GRK5 (GRK5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWZL Neo Myr Flag MAP3K7

Plasmid

#20520

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP3K7 (MAP3K7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Q356H

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Publication

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET14b – E. coli Exonuclease I

Plasmid

#104552

Purpose

bacterial expression of Exonuclease I

Depositor

Charles Bell

Publication

Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

Insert

Exonuclease I (sbcB E.coli)

Tags

6X-His tag followed by thrombin site

Expression

Bacterial

Promoter

T7

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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35S-GWY-GAL4BD/pAM

Plasmid

#201231

Purpose

Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

Depositor

Michael Ayliffe

Publication

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GWY-GAL4BD

Expression

Plant

Promoter

CaMV 35S

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-GFP-HRP-TM_pLX304

Plasmid

#202021

Purpose

expresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vector

Depositor

Alice Ting

Publication

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-GFP-HRP-TM

Use

Lentiviral

Tags

Flag and GFP

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-wtGATA6-3XAU1 puro

Plasmid

#72607

Purpose

Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

Depositor

Kevin Janes

Publication

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

Plasmid

#138145

Purpose

Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

Depositor

Neville Sanjana

Publication

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NLS

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GFPsm-myc.WPRE.SV40

Plasmid

#98926

Purpose

AAV expression of GFP spaghetti monster myc construct

Depositor

Loren Looger

Publication

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

GFP-smFP-myc

Use

AAV

Expression

Mammalian

Promoter

CBA

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158990

Purpose

Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

Depositor

Chung Tin

Publication

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pCMV

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pWZL Neo Myr Flag SGK

Plasmid

#20628

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

SGK (SGK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article