164,728 results

1. pAAV-CAG-SomaGCaMP6f2 (AAV8)

   Viral Prep

   #158757-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Article

   Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

   Promoter

   CAG promoter

   Tags

   None

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

2. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

   Plasmid

   #217635

   Purpose

   AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

   Depositor

   Martin Kampmann

   Article

   Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

   Insert

   mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha and mU6

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Chromoprotein kit 1.0

   Plasmid Kit

   #1000000174

   Purpose

   A synthetic biology collection of plasmids for expression of chromoproteins in bacteria, which can be used as an educational resource.

   Depositor

   Anthony Forster

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pLENTI_RPS10

   Plasmid

   #127129

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   RPS10 (RPS10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   K24R- please see depositor comments

   Promoter

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p206-Switch-ON

   Plasmid

   #217885

   Purpose

   Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRESCA-syntide

   Plasmid

   #132415

   Purpose

   FRET-based sensor for CaMKII activity

   Depositor

   Margaret Stratton

   Article

   Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

   Insert

   FRESCA-syntide
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAG.(cyto).cpSFGFP.HaloTag

   Plasmid

   #209654

   Purpose

   Non-responsive control

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   cpSFGFP.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBP-T7_RBS-His6-Thrombin

   Plasmid

   #72979

   Purpose

   T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7+RBS+His6+thrombin
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

   Plasmid

   #209652

   Purpose

   Expression of iATPSnFR2, medium affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95K.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95K

   Promoter

   CAG

   Available since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Human Brunello kinome pooled library, guides 1-8 in lentiCRISPRv2 backbone

    Pooled Library

    #1000000083

    Purpose

    CRISPR gRNA knockout pooled library for targeting the human kinome

    Depositor

    John Doench, David Root

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only