We narrowed to 3,497 results for: actin

1. pBa.GFP-KIF5B

   Plasmid

   #134625

   Purpose

   Mammalian expression of mouse KIF5B 376-963

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 377 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZac2.1 gfaABC1D-LifeAct-GFP

   Plasmid

   #190199

   Purpose

   Expresses the small actin binding peptide, LifeAct, with a GFP reporter

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   LifeAct-eGFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   gfaABC1D

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

   Plasmid

   #52889

   Purpose

   "CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

   Depositor

   Iswar Hariharan

   Article

   Bosch et al Development. 2015 Feb 1;142(3):597-606.

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pclbw-eGFPomp25

   Plasmid

   #58424

   Purpose

   Mammalian expression of GFP targeted to the mitochondrial outer membrane

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   eGFP-omp25 (Synj2bp Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains aa's 109-145 of mouse omp25

   Promoter

   CMV/b-actin

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCI H2B-RFP

   Plasmid

   #92398

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   IRES H2B RFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Cas13d-T2A-GFP(1-10)

   Plasmid

   #224566

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   Cas13d-T2A-GFP(1-10)
   

   Use

   CRISPR

   Tags

   T2A-split GFP(1-10)

   Expression

   Mutation

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI H2B-GFP

   Plasmid

   #92399

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   IRES H2B EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1-GA_CAG-ITPKA-3x-mScarlet-I

   Plasmid

   #229782

   Purpose

   Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   3x mScarlet-I
   

   Use

   Synthetic Biology

   Tags

   ITPKA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. E407V Nuclear Myosin I YFP

   Plasmid

   #60242

   Purpose

   Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   E407V

   Promoter

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAct:dCas9-GCN4

    Plasmid

    #78899

    Purpose

    Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-10XGCN4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Lifeact-μDpTT

    Plasmid

    #182322

    Purpose

    Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Lifeact-μDronpa-TagRFP-T
    

    Use

    Tags

    N-terminal 17 amino acids from budding yeast Abp1…

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEos4b-Lifeact-7

    Plasmid

    #54813

    Purpose

    Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson, Loren Looger

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mEos4b-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pIS1-Actb5UTR-renilla

    Plasmid

    #38234

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Actb 5'UTR / Renilla (Actb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Actb 5' UTR fused to renilla ORF

    Promoter

    Endogenous Actb

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

14. mEos2-FilaminA-N-9

    Plasmid

    #57379

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    FilaminA (FLNA Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    D2626H

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pExpreS2-1

    Plasmid

    #175360

    Purpose

    ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)

    Plasmid

    #53959

    Purpose

    allows Cre-mediated expression of transgene

    Depositor

    Jeffrey Robbins

    Article

    Nakamura et al Proc Natl Acad Sci U S A. 2009 Sep 8;106

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Lox-CAT-Lox

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer, chicken actin promoter

    Available since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pAct:dCas9-VP64

    Plasmid

    #78901

    Purpose

    Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pR008_APAD

    Plasmid

    #204818

    Purpose

    Expression Apobec1-ADAR2dd fusion protein as PIE-RBP control

    Depositor

    Xiaochang Zhang

    Article

    Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

    Insert

    rat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
    

    Use

    Tags

    HA and Flag and huADAR2dd

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBa-FRB-3myc-Rab5a

    Plasmid

    #64209

    Purpose

    Rab5a with N-terminal FRB and triple myc tags

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Rab5a (Rab5a Mouse)
    

    Use

    Tags

    3myc and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    chicken Beta Actin

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB.FRB-3myc-KLC1a

    Plasmid

    #134627

    Purpose

    Mammalian expression of mouse KLC1a

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KLC1a (Klc1 Mouse)
    

    Use

    Tags

    FRB, 3myc

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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