Addgene

We narrowed to 4,404 results for: ARA-2

Showing: 1721 - 1740 of 4404 results

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pPKm-241

Plasmid

#104855

Purpose

pSIN-cytosFD-P2A-cytosFNR, expressing Synechococcus sp Fd and FNR, under EF-1alpha promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

cytosFd-cytosFNR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

Availability

Academic Institutions and Nonprofits only

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GFP-cCGNL1-HA

Plasmid

#236517

Purpose

For expression of Paracingulin (dog) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (CGNL1 Canis lupus familiaris (dog))

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGNL1

Plasmid

#236519

Purpose

For expression of Paracingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (Cgnl1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-cka

Plasmid

#195067

Purpose

Expresses FLAG-tagged Drosophila cka protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Connector of kinase to AP-1 (Cka Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Ubi-p63e

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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AtCOPT2 uPIC

Plasmid

#117900

Purpose

protein expression

Depositor

Geoffrey Chang

Insert

AtCOPT2 uPIC (COPT2 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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SLAH2

Plasmid

#117104

Purpose

protein expression in yeast

Depositor

Geoffrey Chang

Insert

SLAH2 (SLAH2 Mustard Weed)

Use

Tags

His

Expression

Yeast

Mutation

Promoter

AOX

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Cas9/pTREX-n

Plasmid

#68708

Purpose

Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Insert

Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .

Use

CRISPR

Tags

Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

Expression

Mutation

C4239T mutation that eliminates a BamHI restricti…

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Strep-His-Tev-hCGNL1

Plasmid

#236520

Purpose

For expression of Paracingulin (human) in insect cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (CGNL1 Human)

Use

Tags

2xStrep-10xHis-Tev

Expression

Insect

Mutation

WT

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGNL1-Myc

Plasmid

#236516

Purpose

For expression of Paracingulin (dog) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (CGNL1 Canis lupus familiaris (dog))

Use

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_CEACAM6-His

Plasmid

#149337

Purpose

for eukaryotic expression and purification of CEACAM6-His6

Depositor

James Fleckenstein

Article

Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

Insert

CEACAM6 (CEACAM6 Human)

Use

Tags

IL-2 signal peptide and polyhistidine

Expression

Mammalian

Mutation

point mutation (GGA to GCA) to replace 'G-g…

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2-mCherry

Plasmid

#129716

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSB90 - pAGM4723_VirGN54D

Plasmid

#123187

Purpose

Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Type

Empty backbone

Use

Level 2 acceptor vector for expression in plants

Tags

Expression

Plant

Mutation

VirGN54D for increase virulence

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-HP1alpha

Plasmid

#179703

Purpose

visualization of centromere heterochromatin

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

heterochromatin protein alpha (CBX5 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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dmBACCS2-IRES-dOrai-IRES-mCherry

Plasmid

#72894

Purpose

Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2-IRES-dOrai-IRES-mCherry

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

Plasmid

#197885

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG

Plasmid

#132685

Purpose

AAV plasmid encoding mouse CNDP2 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Carnosine dipeptidase 2 (Cndp2 Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

Chicken b-actin

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ULK2

Plasmid

#97075

Purpose

Expresses ULK2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

ULK2 (ULK2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

Plasmid

#197893

Purpose

Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

EF1a

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4

Plasmid

#157799

Purpose

Expression of mEGFP and human linker histone H1.4 fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4 (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

Plasmid

#160140

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver, Keith Joung

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

NLS(nucleoplasmin)-3xHA-P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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