We narrowed to 5,029 results for: FRI;

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EGFP-miSFIT-10-G, 15-C

Plasmid

#124695

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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C-term png in pGEX4

Plasmid

#112975

Purpose

For protein expression and purification of the C-term of Drosophila png

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png (png Fly)

Expression

Bacterial

Mutation

wild-type C terminus

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVscCBPITuDlet-7Gpluc7xlet7BT

Plasmid

#35651

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD let-7

Use

AAV

Promoter

U6

Available Since

May 7, 2012

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-TuDmiR122 seedonly

Plasmid

#35667

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD miR-122seedonly

Promoter

U6

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pLI_C-tag TNF

Plasmid

#171178

Purpose

inducible expression of C-tag TNF

Depositor

Veit Hornung

Article

Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

Insert

C-tag TNF

Use

Lentiviral

Tags

C-tag (internal)

Expression

Mammalian

Mutation

ADAM cleavage site of TNF (AA73-76) was exchanged…

Promoter

tight TRE promoter

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-D4H*-mCherry

Plasmid

#134604

Purpose

fluorescent cholesterol-binding probe

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

D4H*

Tags

GST and mCherry

Expression

Bacterial

Mutation

Y415A and A463W

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Cdc42-2G

Plasmid

#68814

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Tags

His and Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pHCMV_SARS-CoV-2_Sdel18

Plasmid

#207307

Purpose

Mammalian expression of SARS2 coronavirus S protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SARS-CoV2_S (S SARS2 coronavirus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBS1C

Plasmid

#55168

Purpose

Empty vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pBS4S

Plasmid

#55170

Purpose

Empty vector, integration at thrC, ampr, specr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pCS2-Opto-Acvr1ba-P2A-Acvr2ba

Plasmid

#232640

Purpose

Optogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expression

Depositor

Katherine Rogers

Article

Iannucci et al bioRxiv [Preprint]. 2025 Apr 19:2025.04.

Insert

Acvr1ba + VfLOV domain and Acvr2ba + VfLOV domain

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-DogCatcher

Plasmid

#171929

Purpose

Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

Cys-DogCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K27

Plasmid

#22902

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K27 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K29

Plasmid

#22903

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K29 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K6

Plasmid

#22900

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K6 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K11

Plasmid

#22901

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K11 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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Pveg

Plasmid

#55173

Purpose

Very strong constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

veg promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

veg

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-C

Plasmid

#118367

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-FLAG

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-N

Plasmid

#118366

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-Flag

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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