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pMaCTag-P30

Plasmid

#120041

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

VSV-G

Use

Pcr template

Tags

VSV-G

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-cat_TU

Plasmid

#127051

Purpose

Basic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Cat_TU

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0032-MV_RN

Plasmid

#127015

Purpose

Basic Part RN- RBS+N-terminus; B0033m Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0032m-MV_RN

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-min2

Plasmid

#126956

Purpose

"Minimal cargo" vector. Allows introducing any part using BsmBI; but doesn't have cloning reporter. Can be used to generate new promoter/terminator acceptor vectors.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-25

Plasmid

#120004

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-19

Plasmid

#124787

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDD401

Plasmid

#91828

Purpose

Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Pmyo-2::GFP

Use

CRISPR

Expression

Worm

Mutation

Nucleotide 489 of the myo-2 promoter was changed …

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P03

Plasmid

#120014

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT3A

Plasmid

#79501

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3A (WNT3A Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDISP1

Plasmid

#31235

Depositor

Cliff Ragsdale

Article

Agarwala et al Dev Biol. 2005 Dec 1. 288(1):206-20.

Insert

DISP1 (DISP1 Chicken)

Use

Cdna for in situ hybridization

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only
pEN_TmiR_G13-L

Plasmid

#25762

Purpose

Entry vector with TRE promoter driving mouse G alpha 13 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pSRE_G

Plasmid

#240260

Purpose

Circuit-responsive (SRE promoter) expression of vesicular stomatitis virus G protein (VSVG). Used to produce envelope protein for PROTEUS VLVs during SRF-VP64 campaigns.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

VSV-G

Expression

Mammalian

Promoter

SRE

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTRE3G_G

Plasmid

#240261

Purpose

Circuit-responsive (TRE3G promoter) expression of vesicular stomatitis virus G protein (VSVG). Used to produce envelope protein for PROTEUS VLVs during tTA/rtTA-3G campaigns.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

VSV-G

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pZF43x6-C_G

Plasmid

#240262

Purpose

Circuit-responsive (ZF43RE promoter) expression of vesicular stomatitis virus G protein (VSVG). Used to produce envelope protein for PROTEUS VLVs during Nb139-VP64 campaigns.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

VSV-G

Expression

Mammalian

Promoter

ZF43x6-C

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_TRE3G_luciferase

Plasmid

#240266

Purpose

Circuit-responsive (TRE3G promoter) expression of firefly luciferase. Used for evaluation of tTA/rtTA-3G transgene activity.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pANC79b(P3-Myc-nTEV-IRES-EBFP)

Plasmid

#248357

Purpose

Lentiviral vector that can express P3 along with N terminal split TEV. Expressed off of CMV tet ON promoter with EBFP marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

P3-Myc-nTEV

Use

Lentiviral

Promoter

tight TRE

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pANC79a(P4-GGGSGGG-cTEV-HA-IRES-dTomato)

Plasmid

#248356

Purpose

Lentiviral vector that can express P4 along with C terminal split TEV. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

P4-GGGSGGG-cTEV-HA

Use

Lentiviral

Promoter

tight TRE

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pCSCG-Fc-pST3Gal1 H302A

Plasmid

#228820

Purpose

Expresses Fc-fusion pST3Gal1 H302A in mammalian cells

Depositor

Sriram Neelamegham

Article

Hombu et al Nat Commun. 2025 Jul 18;16(1):6637. doi:

Insert

Fc-pST3Gal1 H302A

Tags

6His tag and human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-59 of pST3Gal1 were deleted and H30…

Promoter

CMV

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pANC76(TVMVp-IRES-dTomato)

Plasmid

#248362

Purpose

Lentiviral vector that can express TVMVp. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

TVMVp

Use

Lentiviral

Promoter

tight TRE

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pNTA-uTEV3-GSG-t2A-EBFP

Plasmid

#248360

Purpose

Lentiviral vector that can express uTEV3. Expressed off of CMV tet ON promoter with EBFP marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

uTEV3-GSG-t2A-EBFP

Use

Lentiviral

Promoter

tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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