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pGPI-SceI
Plasmid#32060PurposeConstruction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of BurkholderiaDepositorTypeEmpty backboneUseMutagenesis plasmidTagsExpressionMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS2-2
Plasmid#220628PurposePlasmid allows you to place the RNA sequence of interest next to two MS2 coat protein recognition sites.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCMycDam
Plasmid#59218PurposeDrosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli DamDepositorTypeEmpty backboneUseDamidTagsDam and MycExpressionInsectMutationPromoterDrosophila heat-shock promoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIHEU-MCS
Plasmid#58375PurposeUAS vector to express gene of interest in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
p405MET25
Plasmid#15961DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Myc
Plasmid#119757PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.DepositorTypeEmpty backboneUseTagsGST and MycExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFKBPmCherry-Akt2(KD)
Plasmid#86598PurposeExpresses recruitable mCherry-tagged kinase domain of human Akt2(WT)DepositorInsertFKBP-mCherry-Akt2(KD)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRL8
Plasmid#24980DepositorInsertCbr-unc-119
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHaloTag-PS(RA)
Plasmid#86627PurposeExpresses Akt pseudosubstrate (P-3 RA) mutant tagged with a HaloTagDepositorInsertHaloTag-PS(RA)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
H-ACNF-H
Plasmid#15515DepositorInsertH element
UseMouse TargetingTagsExpressionMutationTargeting vector to delete the 2,119 nt sequence,…PromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX-HA(AscI)
Plasmid#15404DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutation700 bp insert can be released by digestion with r…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDL1530
Plasmid#226636PurposeThis plasmid is for the induced expression of nrsB (formerly known as ORF229) from ICP1_2001.DepositorInsertnrsB (ORF 229)
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1400
Plasmid#213121PurposeTesting the activity of BpiI-associated switch methylasesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1401
Plasmid#213122PurposeTesting the activity of LguI-associated switch methylasesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1399
Plasmid#213120PurposeTesting the activity of BsaI-associated switch methylasesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP
Plasmid#21373Purposeself-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCL20C-ddIPpoI
Plasmid#49053PurposeExpresses the restriction enzyme IPpoI under the control of Shield1DepositorInsertDD-HA-ER-IPpoI
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pABY-T2
Plasmid#201765PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only
