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pC001 - huLshC2C2-MBP for bacterial expression

Plasmid

#79150

Purpose

Expresses human codon-optimized LshC2c2 for purification in E. coli

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

C2c2

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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AgDD

Plasmid

#78289

Purpose

create protein aggregates in living cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NES-L106P-sfGFP (FKBP1A Human)

Tags

MLALKLAGLDI

Expression

Mammalian

Promoter

pCAG

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLHCX-FLAG-mPKM2

Plasmid

#44239

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

Insert

Pyruvate Kinase M2 (Pkm Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pEThT-MAHS delta MTP

Plasmid

#90032

Purpose

To express His-tagged MAHS in bacteria

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

MAHS

Tags

His

Expression

Bacterial

Mutation

Deletion of mitochondria targeting peptide

Promoter

T7

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SHP2 WT

Plasmid

#8329

Depositor

Ben Neel

Article

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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Lyn-FKBP-FKBP-CFP (LF2C)

Plasmid

#20149

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

Lyn-FKBP-FKBP-CFP (FKBP1A Human)

Expression

Mammalian

Mutation

See depositor comments below.

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pAAVTRE3_tdTomato

Plasmid

#135178

Purpose

AAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling

Depositor

Tetsuo Yamamori

Article

Abe et al Front Neuroanat. 2018 Oct 30;12:89. doi:

Inserts

tdTomato
TRE3G

Use

AAV

Expression

Mammalian

Mutation

a variant of DsRed

Promoter

TRE3G

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_AHR

Plasmid

#101071

Purpose

Donor vector for 3' FLAG tag of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

AHR homology arms (AHR Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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keratin 8 (krt8)-H2B-RFP

Plasmid

#105955

Purpose

transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

krt8 (KRT8 Human)

Tags

RFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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His-PIMT

Plasmid

#34852

Depositor

Bruce Downie

Article

Dinkins et al Plant J. 2008 Jul;55(1):1-13.

Insert

PIMT (PCMT1 Human)

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
CMV BARK D110A p2A mCherry

Plasmid

#117690

Purpose

CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Tags

HA / FLAG

Expression

Mammalian

Mutation

D110A

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCMMP-BiP-IRES-mRFP

Plasmid

#36975

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Insert

BiP (HSPA5 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

Plasmid

#52889

Purpose

"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

Depositor

Iswar Hariharan

Article

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast

Plasmid

#167271

Purpose

Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-HA-CYLD

Plasmid

#22544

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

CYLD (CYLD Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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LifeAct-14 (LA-14)

Plasmid

#158750

Purpose

Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

Depositor

Minhajuddin Sirajuddin

Article

Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

Insert

LifeAct-14

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Deletion mutation for last three amino acids from…

Promoter

CMV immediate early

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGEXtev-HMG54kd

Plasmid

#26030

Depositor

Johann Deisenhofer

Article

Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

Insert

3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)

Tags

GST

Expression

Bacterial

Mutation

Catalytic domain (amino acids 426-488) Methion…

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pLHCX-FLAG-mPKM1

Plasmid

#44240

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

Insert

Pyruvate Kinase M1 (Pkm Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_GNAI1_G-alpha

Plasmid

#110012

Purpose

Protein expression and purification of GNAI1_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAI1_G-alpha (GNAI1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-CYLD-STOP

Plasmid

#60077

Purpose

N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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