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We narrowed to 7,588 results for: Lif;

Showing: 1741 - 1760 of 7588 results

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pUE1-TSP-ORF68 R443A

Plasmid

#162652

Purpose

Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acid R443 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DFO-ChR2(H134R)-EYFP-WPRE

Plasmid

#136916

Purpose

Expresses ChR2(H134R)-EYFP in Cre-negative cells

Depositor

Ofer Yizhar

Article

Sermet et al Elife. 2019 Dec 20;8. pii: 52665. doi: 1

Insert

ChR2(H134R)-EYFP

Use

AAV

Tags

Expression

Mutation

H134R in ChR2

Promoter

Human Synapsin1

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Article

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEF NS34a-msGFP-'S-mito

Plasmid

#158765

Purpose

Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

NS3/4a protease and msGFP

Use

Tags

Expression

Mammalian

Mutation

Flexible linkers between the protease and msGFP a…

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Mito-CaNARi

Plasmid

#64851

Purpose

Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Use

Tags

Cerulean, N-terminal 30 amino acids from DAKAP1, …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMVtight-RFP-IRES-CD

Plasmid

#126678

Purpose

Doxycycline inducible expression of CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

RFP-IRES-CD (FCY1 Budding Yeast)

Use

Tags

Expression

Mutation

Promoter

tight TRE

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pXWJ109HgAmp30E

Plasmid

#123149

Purpose

Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J109, PmerT, PhrpLE

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX2T VPS4B, residues 1-86

Plasmid

#180639

Purpose

Bacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

VPS4B (VPS4B Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Residues 1-86

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-tdTomato-ChIEF

Plasmid

#172370

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

tdTomato + ChIEF

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Plasmid

#125225

Purpose

Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pT2ADW-lox-mCherry-hyBRET-ERK-NLS

Plasmid

#170290

Purpose

For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

lox-mCherry-lox-hyBRET-ERK-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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mcherry-p62 Double Mutation/Silent

Plasmid

#187982

Purpose

K7A/D69A p62 double mutant (PB1AA). Internal reference: SMC532

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

D7A, D69A, silent mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3456

Plasmid

#179581

Purpose

Pnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

BFP

Expression

Worm

Mutation

Promoter

Pnmr-1

Available since

March 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCL1316

Plasmid

#217958

Purpose

Expression of human H4 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H4 (H4C1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Dead

Plasmid

#173073

Purpose

Dead SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Use

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Mutation

AA144 in both SNAP tags changed from a cysteine t…

Promoter

CMV IE94

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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All-in-one split-ADAR2

Plasmid

#170136

Purpose

AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-POLD3

Plasmid

#183197

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-POLD3

Use

CRISPR; Gateway entry clone

Tags

Expression

Mutation

n/a

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2pA2-drl:APEX2-mCherry

Plasmid

#188945

Purpose

Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

Depositor

Owen Tamplin

Article

Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

Insert

drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA

Use

Tol2 destination vector (attr4-r3)

Tags

Expression

Mutation

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

Plasmid

#129537

Purpose

PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

Depositor

Hideyuki Okano

Article

Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

Insert

tetR-3xFlag-HA-2A-Puro

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

hPGK promoter

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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