We narrowed to 8,109 results for: URE

Showing: 1741 - 1760 of 8109 results

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pPICZc-HsACTG1*-thymosinB-8His

Plasmid

#111147

Purpose

Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

actin gamma 1 (ACTG1 Human)

Use

Pichia pastoris integration vector

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

codon-optimised for expression in Pichia pastoris

Promoter

AOX1

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-G9aCD

Plasmid

#235580

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector G9a fused with scFV to programme H3K9me2/3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

G9a (Ehmt2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pMH4-SYN-ChR2-tdimer2RFP

Plasmid

#22772

Depositor

Thomas Oertner

Article

Zhang et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

ChR2-tdimer2RFP

Tags

ChR2-tdimer2RFP

Expression

Mammalian

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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HMA-APOBEC1-Nb

Plasmid

#226012

Purpose

For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

Depositor

Eugene Yeo

Article

Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

Insert

APOBEC1 (Apobec1 Rat)

Tags

His-MBP and antiRabbit-nanobody

Expression

Bacterial

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4/2

Plasmid

#71712

Purpose

Membrane scaffold protein (MSP), half of helix 4 truncated

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDF0387 pcmv-hudiscas7-11-r978-gggs-r1294

Plasmid

#186984

Purpose

mammalian expression of cas7-11-r978-gggs-r1294

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r978-gggs-r1294

Expression

Mammalian

Mutation

r978-gggs-r1294 deletion

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-DHX36iso1E335A

Plasmid

#159587

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

E335A

Promoter

CMV

Available Since

May 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-MjDim1

Plasmid

#189936

Purpose

Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

Depositor

Chuan He

Article

Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

Insert

rsmA (MJ_RS00455 Methanocaldococcus jannaschii)

Tags

6x His tag, FLAG tag, SUMO tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-EGFP-RIP2

Plasmid

#131201

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 (RIPK2 Human)

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP-pk-S65A

Plasmid

#115619

Purpose

Expresses human Parkin S65A in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

S65A

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcold-6xHis-HRV3Csite-HsTRBP

Plasmid

#41096

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

TRBP (TARBP2 Human)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pet26-MBP-ELIC

Plasmid

#39239

Depositor

Raimund Dutzler

Article

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

Eriwinia chrysanthemi - pentameric ligand gated ion channel

Tags

His-10 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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MPZ-intron1 pGL3-TATA

Plasmid

#21630

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

myelin protein zero intron1 (Mpz Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)

Plasmid

#85799

Purpose

donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Mutation

homology arms for knock-in into first intron

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCnCas12f1HS_sgRNA_MS13_empty

Plasmid

#204999

Purpose

CnCas12f1 with MS13 sgRNA site for Human cell genome editing

Depositor

Quanjiang Ji

Article

Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTK203

Plasmid

#13536

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-TCTY

Tags

See map

Expression

Bacterial

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PI4K2A

Plasmid

#221760

Purpose

Stable expression of GFP-PI4K2A in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PI4K2A (PI4K2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-Hygro-2A-TMRPSS2

Plasmid

#170390

Purpose

Generation of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cells

Depositor

Nir Hacohen

Article

Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

June 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-parkin 77-465

Plasmid

#45970

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 77-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1-v5.1+T1-empty

Plasmid

#204977

Purpose

AsCas12f1-v5.1+T1-based Human cell genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nature Catalysis (2023) 6, 695–709

Type

Empty backbone

Use

CRISPR

Mutation

N70Q/K103R/A104R/S118A/D364R

Promoter

PCMV,U6

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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