We narrowed to 51,423 results for: son

1. pCJ202

   Plasmid

   #162675

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   W159C, S238C; codon optimized for expression in E…

   Promoter

   T7/lac

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCJ199

   Plasmid

   #162672

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCJ197

   Plasmid

   #162670

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   S131G; codon optimized for expression in E. coli …

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCJ203

   Plasmid

   #162678

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   deltaF2-A75; codon optimized for expression in E.…

   Promoter

   T7/lac

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-iRFP-STOP-ReNL

   Plasmid

   #113965

   Purpose

   Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)

   Depositor

   Jordan Green

   Article

   Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

   Inserts

   iRFP670
   ReNL
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   CMV - SV40-PolyA - SV40-PolyA and EF1A

   Available Since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MDM4_Deletion_Downstream_gRNA_2

   Plasmid

   #195137

   Purpose

   gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 Deletion Downstream gRNA 2 (MDM4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. MDM4_CRISPRi_gRNA_2

   Plasmid

   #195133

   Purpose

   gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 CRISPRi gRNA 2 (MDM4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MDM4_CRISPRi_gRNA_1

   Plasmid

   #195132

   Purpose

   gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 CRISPRi gRNA 1 (MDM4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MDM4_Deletion_Downstream_gRNA_1

   Plasmid

   #195136

   Purpose

   gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 Deletion Downstream gRNA 1 (MDM4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCJ205

    Plasmid

    #162676

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C224A, C529A; codon optimized for expression in E…

    Promoter

    T7/lac

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCJ198

    Plasmid

    #162671

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    F495I; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCJ196

    Plasmid

    #162669

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S225A; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCJ204

    Plasmid

    #162677

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C224H, C529F; codon optimized for expression in E…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCJ201

    Plasmid

    #162674

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C224W, C529SS; codon optimized for expression in …

    Promoter

    T7/lac

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mKusabira Orange-N1

    Plasmid

    #55046

    Purpose

    Empty backbone for fusing gene of interest to N-term of mKO

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mKO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCJ190

    Plasmid

    #162667

    Purpose

    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCJ217

    Plasmid

    #162685

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    G489C, S530C; codon optimized for E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRRL-MND-hCard11 WT-T2A-GFP

    Plasmid

    #163787

    Purpose

    Expression of Wild-type CARD11

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Promoter

    MND

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7NT*-Bri2 113-231 R221E

    Plasmid

    #138134

    Purpose

    Expresseds human Bri2 BRICHOS R221E mutant in E. coli

    Depositor

    Jan Johansson

    Article

    Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

    Insert

    human Bri2 BRICHOS R221E (ITM2B Human)
    

    Tags

    NT* tag derived from spider silk proteins

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCJ189

    Plasmid

    #162666

    Purpose

    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge