We narrowed to 31,787 results for: son
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Plasmid#155220PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
TagsmCitrineExpressionMammalianAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV CMV GCGR-V5-sTurboID (N)
Plasmid#153009PurposeExpresses split-TuboID N-terminal fragment fused to GCGRDepositorInsertGCGR-sTurboID (N) (L73)
UseAAVTagsV5ExpressionMammalianPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS6-YFP-SMASh(AI)
Plasmid#111500PurposeExpresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYFP-SMASh(AI)
TagsHA epitope tag and SMASh tagExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRBC-sfGFP wild type
Plasmid#174075PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
TagsHis-6ExpressionBacterialPromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-flex-Voltron
Plasmid#119035PurposeCre-dependant expression of Voltron in neuronsDepositorInsertVoltron
UseAAVExpressionMammalianPromoterhsynAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
His6-pAG-RTase
Plasmid#214932PurposeExpress fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systemsDepositorInsertfusion protein of protein A/G and MMLV reverse transcriptase (25-497)
TagsHis6ExpressionBacterialMutationMMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…PromoterT7 promoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTOX4
Plasmid#127449PurposeEncodes YhaV toxin and amilCP chromoprotein. CAM-RDepositorInsertsyhaV toxin
amilCP chromoprotein
ExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEP-LAMP1-RFP
Plasmid#167970PurposeExpresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMP1 (Lamp1 Rat)
TagsSEP and mRFPExpressionMammalianMutationD50EPromoterCMVAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEJS1089: mini-AAV.sgRNA.Nme2Cas9
Plasmid#159536PurposeDelivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 KbDepositorInsertNme2Cas9 with single guide RNA cassette
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 asyn E46K
Plasmid#105730PurposeMammalian expression of mutant human alpha synucleinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha-synuclein E46K (SNCA Human)
ExpressionMammalianMutationE46KPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA6 asyn A30P
Plasmid#105729PurposeMammalian expression of mutant human alpha synucleinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha-synuclein A30P (SNCA Human)
ExpressionMammalianMutationA30PPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1(TATV)(BB) (pY221)
Plasmid#89354PurposeExpresses humanized AsCpf1 TATV PAM variant and crRNA guideDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCpf1 RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCBhAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTag-eGFP-SV40
Plasmid#117813PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
UseSynthetic BiologyAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_GATA6_P2A_Hygro_Barcode
Plasmid#120445PurposeBarcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgRNAtet-lvaA
Plasmid#106397PurposeGuide RNA plasmid targeting lvaA on BBR1-UP originDepositorInsertsgRNA towards lvaA in Pseudomonas putida
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
CGBE1-NG (pBM1246)
Plasmid#140256PurposeCMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteUNG-BE4max(R33A)_NG-ΔUGI
ExpressionMammalianMutationR33A in rAPOBEC1, NG mutations in SpCas9, D10A in…PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaQ-RLuc8
Plasmid#140982PurposeEncodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlphaQ-RLuc8 (GNAQ Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDNA2.0 6H-TEV-KRAS G12V
Plasmid#111850PurposeExpresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G12V (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 12 to ValinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAJM.677
Plasmid#108530PurposeAraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.DepositorInsertAraCAM + AraE + PBAD-YFP
ExpressionBacterialMutationAraC: L133F E165L E169G C280LAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AsCpf1-2NLS
Plasmid#102565Purposebacterial expression of AsCpf1-2NLSDepositorInsertAsCpf1
Tags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by