We narrowed to 20,088 results for: Kin

1. pSICO-CPSF6-iRFP670

   Plasmid

   #167586

   Purpose

   Expresses human CPSF6 fused to iRFP

   Depositor

   Zandrea Ambrose

   Article

   Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

   Insert

   CPSF6-iRFP670 (CPSF6 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mutation

   WT

   Promoter

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJH126

   Plasmid

   #162482

   Purpose

   SEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

   Depositor

   Bob Goldstein

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
   

   Use

   CRISPR

   Tags

   vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pESC-LEU-SopB

   Plasmid

   #183670

   Purpose

   Inducible Salmonella Typhimurium SopB for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pESC-Leu-SopB(C460S)

   Plasmid

   #183671

   Purpose

   Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C460S

   Promoter

   GAL1

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HtrA2-FLAG S400D

   Plasmid

   #15940

   Depositor

   L. Miguel Martins

   Article

   Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S400D

   Promoter

   Available Since

   Nov. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA5 FRT TO GFP MPS1 K-R

   Plasmid

   #59819

   Purpose

   Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

   Insert

   Mps1 (TTK Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   KD, catalytically inactive, D664A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLAP-BUBR1-dKARD

   Plasmid

   #114053

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1-dKARD (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

   Promoter

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR3-FLAG-Gephyrin G

   Plasmid

   #68817

   Purpose

   Geph G domain expression in mamalian cells

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   NIL

   Promoter

   CMV

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

9. His6-SMT3-SopB

   Plasmid

   #183676

   Purpose

   Recombinant Salmonella Typhimurium SopB

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   His6-S. cerevisiae SMT3(2-101)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5 Flag p38 alpha

    Plasmid

    #20786

    Depositor

    Roger Davis

    Article

    Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

    Insert

    pCMV5 Flag p38 alpha (Mapk14 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pCR3-Flag-AuroraB KD (K106R)

    Plasmid

    #108488

    Purpose

    expression of Flag-AuroraB kinase death (K106R)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Kinase death K106A

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTD74

    Plasmid

    #172898

    Purpose

    Muscle promoter unc-54P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on chromosome I

    Depositor

    David Reiner

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    TIR-1 (TIR1 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CSK_HUMAN_D0

    Plasmid

    #79683

    Purpose

    This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSK (CSK Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3.1-nAChr-beta3

    Plasmid

    #48811

    Purpose

    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

    Depositor

    Jerry Stitzel, Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTD82

    Plasmid

    #172903

    Purpose

    Muscle promoter unc-54P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

    Depositor

    David Reiner

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    TIR-1 (TIR1 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTD75

    Plasmid

    #172899

    Purpose

    Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on LGI

    Depositor

    David Reiner

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    TIR-1 (TIR1 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HtrA2-FLAG S142D

    Plasmid

    #15939

    Depositor

    L. Miguel Martins

    Article

    Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S142D

    Promoter

    Available Since

    Nov. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pJW1927

    Plasmid

    #163093

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX459v2-ILK-gRNA 2-exon 8

    Plasmid

    #163321

    Purpose

    SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 2_Exon8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6F

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pAN19-3xFLAG-TIR1-NOSt

    Plasmid

    #108545

    Purpose

    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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