We narrowed to 8,147 results for: Lif

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pmCherry-C1-mTrim21

Plasmid

#105516

Purpose

Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-25xERSE2-minP-EGFP

Plasmid

#159667

Purpose

EGFP reporter plasmid containing 25 tandem repeats of the ER stress response element-2 (ERSE2)

Depositor

Seiichi Oyadomari

Article

Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

Insert

25xERSE2-minP-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-Sec61β

Plasmid

#186574

Purpose

Expression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cells

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

SEC61B (SEC61B Human)

Tags

FRB-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Salsa6f

Plasmid

#140188

Purpose

Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

Depositor

Michael Cahalan

Article

Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

Insert

Salsa6f

Tags

tdTomato-v5-GCaMP6f

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125228

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-humanTRIM5-FOS

Plasmid

#79066

Purpose

Express human TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(human)-FOS (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-HygroR-T2A-TetOn3G

Plasmid

#179887

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

Hygromycin resistance

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Sst44-GFP

Plasmid

#172310

Purpose

SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

Depositor

Michael Greenberg

Article

Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

Insert

Sst44

Use

AAV

Promoter

pB-Globin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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Opto-CRAC version 1

Plasmid

#101245

Purpose

Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

Depositor

Yubin Zhou

Article

He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

Insert

LOV2 and STIM1 cytosolic fragment

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

Plasmid

#130665

Purpose

Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

Depositor

Leslie Vosshall

Article

Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

Insert

AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40

Use

Ae. aegypti expression

Expression

Insect

Promoter

Aedes aegypti poly-ubiquitin

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

Plasmid

#167942

Purpose

Inducible NKX2-1 overexpression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

TTF1 (NKX2-1 Human)

Use

Lentiviral

Promoter

tetON

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1

Plasmid

#187925

Purpose

sgRNA vector backbone expressing puromycin resistance and CD90.1 as selection markers

Depositor

Martin Turner

Article

Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

Insert

hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1

Use

CRISPR and Retroviral

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJCC_076 AsCas12a R1226A

Plasmid

#171669

Purpose

For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a R1226A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed arginine 1226 to alanine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR

Plasmid

#126679

Purpose

Fluorescent reporter for cyclin E/A-CDK and CDK4/6 activities

Depositor

Hee Won Yang

Article

Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

Insert

Rb (a.a 886-928)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-tomato-TSC2-sgRNA

Plasmid

#196195

Purpose

Editing human TSC2 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

TSC2 (TSC2 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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psiCHECK2_Rluc-Y0x-Fluc

Plasmid

#233614

Purpose

Expression of Rluc-Fluc reporter gene containing no Y

Depositor

Shintaro Iwasaki

Article

Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

Insert

Rluc-Y0x-Fluc

Use

Luciferase

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMCB306-GFP-Rab12

Plasmid

#198471

Purpose

lentiviral expression of GFP-Rab12

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

GFP-Rab12 (RAB12 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1A

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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Talin Head

Plasmid

#186140

Purpose

For expression of recombinant Talin head domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

TLN1 (TLN1 Human)

Tags

His6 (tev cleavage)

Expression

Bacterial

Promoter

Lac

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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SMT205

Plasmid

#134817

Purpose

SMT201 with mutated RBS for DHFR, in vivo assays

Depositor

Tanja Kortemme

Article

Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

Inserts

Dihydrofolate reductase
Thymidylate synthase

Expression

Bacterial

Mutation

Silent mutations to eliminate internal restrictio…

Promoter

T7 and TET

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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