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GFP-Rab9a

Plasmid

#169071

Purpose

Express human Rab9a in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

acGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP Asn10 TEV LIC cloning vector (1C)

Plasmid

#29654

Depositor

Scott Gradia

Type

Empty backbone

Tags

10xAsn, 6xHis, MBP, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA GNSTM-3-Flag-10-Lamp2b-HA

Plasmid

#71293

Purpose

Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Tags

FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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4xCSL-luciferase

Plasmid

#41726

Depositor

Raphael Kopan

Article

Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

Insert

4xCSL binding sites

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains a multimerized high affinity CSL binding…

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pLOX-TERT-iresTK

Plasmid

#12245

Purpose

2nd generation lentiviral vector for reversible immortalization of human primary cells

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

TERT, HSV1-TK (TERT Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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LCV2_EGFP

Plasmid

#155098

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-EA-evoAPOBEC1max-32NLS_NG-BlastR

Plasmid

#232722

Purpose

C-to-T base editor

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

evoAPOBEC1max H47E+S48A-32NLS linker-nCas9(NG)-UGI-UGI

Expression

Mammalian

Mutation

within evoAPOBEC1: H47E+S48A; within Cas9 D10A

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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p2T-U6-sgCTG

Plasmid

#232723

Purpose

SpCas9 guide RNA targeting CAG repeats for C-to-T base editing

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

SpCas9 gRNA targeting CAG repeats

Expression

Mammalian

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-15

Plasmid

#228971

Purpose

For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-15

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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V245 pCEP-4HA B56alpha

Plasmid

#14532

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)

Tags

4 HA

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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