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Showing: 321 - 360 of 617 results

  1. pDest27-mBIN1+17

    Plasmid
    #61096
    Purpose
    Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells
    Depositor
    Robin Shaw
    Article
    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.
    Insert
    mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. YFVdelNS1-mCherry

    Plasmid
    #179951
    Purpose
    pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry
    Use
    Transneuronal tracing
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Available since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    179951-plasmid-map-sequence-id-354327

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  7. HA-IFITM3-F8-TAG-C72A

    Plasmid
    #122477
    Purpose
    expresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Promoter
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122477-plasmid-map-sequence-id-237728

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  12. IFITM3-N21del-V93-TAG

    Plasmid
    #122480
    Purpose
    expresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Valine 93 to amber codon and deleted amin…
    Promoter
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122480-plasmid-map-sequence-id-237764

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  13. GFP(N-glyc)-Tac-TC

    Plasmid
    #162496
    Purpose
    Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its TMD and cysotol…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162496-plasmid-map-sequence-id-323476

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  14. pHA-N1-GalT

    Plasmid
    #87326
    Purpose
    To express GalT-HA in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-F
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87326-plasmid-map-sequence-id-177295

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  15. HA-IFITM3-F8-TAG-C105A

    Plasmid
    #122478
    Purpose
    expresses human IFITM3-C105A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Promoter
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122478-plasmid-map-sequence-id-237731

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  16. HA-IFITM3-V93-TAG

    Plasmid
    #122479
    Purpose
    expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Valine 93 to amber codon
    Promoter
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122479-plasmid-map-sequence-id-237724

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  17. HA-IFITM3-F8-TAG-C71A

    Plasmid
    #122476
    Purpose
    expresses human IFITM3-C71A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Promoter
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122476-plasmid-map-sequence-id-237722

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  18. pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

    Plasmid
    #58414
    Purpose
    Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells
    Depositor
    Jacob Yount, Howard Hang
    Article
    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41
    Insert
    IFITM3 (Ifitm3 Mouse)
    Use
    Tags
    HA internal
    Expression
    Mammalian
    Mutation
    Cysteines 71, 72, and 105 to Alanine; The sequenc…
    Promoter
    CMV IE
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. ST-Tstem(5A)-GFP

    Plasmid
    #162502
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The mutated Tac stem region is inserted within ST…
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    162502-plasmid-map-sequence-id-323507

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  29. ST-Tstem-GFP

    Plasmid
    #162501
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    ST6Gal1 mutant (Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The Tac stem region is inserted within ST6Gal1
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162501-plasmid-map-sequence-id-323506

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  30. GFP-Tac-STC(5A)

    Plasmid
    #162491
    Purpose
    Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its stem region, TM…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162491-plasmid-map-sequence-id-323469

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  33. pSmart-Nm-sgRNA-BbsI

    Plasmid
    #49157
    Purpose
    Plasmid for cloning spacer into sgRNA for NmCas9
    Depositor
    James Thomson
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    49157-plasmid-map-sequence-id-72446

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  36. pMAL-SH3(2)-5R

    Plasmid
    #112089
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    SH3(2)-5R (NCK1 Human)
    Use
    Tags
    His6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only repeats of the second SH3…
    Promoter
    Lac
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112089-plasmid-map-sequence-id-236082

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  37. WT Smarca4-sfGFP

    Plasmid
    #107056
    Purpose
    Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Courtney Hodges, Jerry Crabtree
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107056-plasmid-map-sequence-id-321500

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  38. WT Smarca4-V5 IRES-puro

    Plasmid
    #107058
    Purpose
    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Courtney Hodges, Jerry Crabtree
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107058-plasmid-map-sequence-id-201960

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  39. pAAV-SynaptoTAG2

    Plasmid
    #175275
    Purpose
    AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    EGFP-Synaptobrevin-2; tdTomato (Vamp2 )
    Use
    AAV
    Tags
    EGFP and tdTomato (P2A cleavage)
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175275-plasmid-map-sequence-id-349084

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  40. FUW-C-prM-E-NS1

    Plasmid
    #175281
    Purpose
    lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP.
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    C-prM-E-NS1 and EGFP (POLY Yellow fever virus strain 17D)
    Use
    Lentiviral
    Tags
    IRES EGFP
    Expression
    Mutation
    Promoter
    human ubiquitin C
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175281-plasmid-map-sequence-id-349091

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Showing: 321 - 360 of 617 results