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Str-KDEL_SBP-EGFP-CCR5

Plasmid

#222309

Purpose

Synchronize the trafficking of CCR5 from the ER.

Depositor

Franck Perez

Article

Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

Insert

Streptavidin-KDEL and CCR5 fused to SBP-EGFP (CCR5 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CCR5-Cys3A

Plasmid

#222313

Purpose

Synchronize the trafficking of CCR5-Cys3A from the ER.

Depositor

Franck Perez

Article

Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

Insert

Streptavidin-KDEL and CCR5-Cys3A fused to SBP-EGFP (CCR5 Human)

Expression

Mammalian

Mutation

C321A, C323A, C324A

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pBiFCt-2in1-CC

Plasmid

#105114

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Tags

cYFP-Myc/nYFP-HA

Expression

Plant

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBiFCt-2in1-NC

Plasmid

#105112

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Tags

cYFP-Myc and nYFP-HA

Expression

Plant

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBiFCt-2in1-CN

Plasmid

#105113

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Tags

cYFP-HA and nYFP-Myc

Expression

Plant

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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IRES-puro-GFP

Plasmid

#16616

Depositor

Bert Vogelstein

Article

Torrance et al Nat Biotechnol. 2001 Oct . 19(10):940-5.

Insert

GFP

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-5FLAG-KanMX6

Plasmid

#15983

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Type

Empty backbone

Tags

5xFlag

Expression

Bacterial

Available Since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pTN182

Plasmid

#34888

Depositor

Mitsuyasu Hasebe

Article

Sakakibara et al Evol Dev. 2008 Sep-Oct;10(5):555-66.

Type

Empty backbone

Use

Moss targeting

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pET29b_C6-Cys-His

Plasmid

#233323

Purpose

To express nanobody C6 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

C6-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_VHH16-Cys-His

Plasmid

#233324

Purpose

To express nanobody VHH16 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

VHH16-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_VHH21-Cys-His

Plasmid

#233325

Purpose

To express nanobody VHH21 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

VHH21-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_1-Cys-His

Plasmid

#233326

Purpose

To express nanobody IGHJ4_1 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_1-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_2-Cys-His

Plasmid

#233327

Purpose

To express nanobody IGHJ4_2 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_2-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_3-Cys-His

Plasmid

#233328

Purpose

To express nanobody IGHJ4_3 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_3-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_4-Cys-His

Plasmid

#233329

Purpose

To express nanobody IGHJ4_4 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_4-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ5-Cys-His

Plasmid

#233330

Purpose

To express nanobody IGHJ5 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ5-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Myo9b R1695M

Plasmid

#134911

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

Depositor

Martin Bähler

Article

Muller et al Mol Biol Cell. 1997 Oct;8(10):2039-53. d

Insert

Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

changed arginine 1695 to methionine

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-LOV-VP16

Plasmid

#42499

Purpose

LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor

Depositor

Ricardo Dolmetsch

Article

Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

Insert

FKF1 (N-LOV, G128D mutant) fused with VP16

Tags

F-type VP16 activation domain and HA tag

Expression

Mammalian

Mutation

G128D 1-168aa

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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GST-MNase

Plasmid

#136291

Purpose

Expresses Micrococcal nuclease

Depositor

Kavitha Sarma

Article

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

MNase

Expression

Bacterial

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pNX32-Dest

Plasmid

#105083

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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