We narrowed to 2,486 results for: EFS

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pAAV-U6-DR30(SapI)-EFS-CasRx-pA

Plasmid

#192488

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-3XNeon-nls

Plasmid

#75386

Purpose

PCP-3XNeon-nls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

PCP

Use

Lentiviral

Tags

3XmNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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hU6-pegRNA-EFS-mScarlet Template

Plasmid

#196037

Purpose

The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

Depositor

Tyler Jacks

Article

Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

Insert

mScarlet

Expression

Mammalian

Promoter

hU6 and EFS

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-3xSNAP-NLS

Plasmid

#236388

Purpose

Overexpression of PCP-3xSNAP in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

PCP-3xSNAP

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pRC10-EFS-huLbCpf1-2A-Blast-WPRE

Plasmid

#123359

Purpose

Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLY099-RetroEFS-BCMABBz-PRODH2-WPRE

Plasmid

#192200

Purpose

Retro-BCMACAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-BCMACAR-PRODH2 (TNFRSF17 Synthetic)

Use

Retroviral; Mammalian expression

Mutation

NA

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP304-pAAV-EFS-dSaCas9-VP64-pA

Plasmid

#113679

Purpose

A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Synthetic Biology

Tags

NLS and VP64

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

EF1a-short; U6

Available Since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
pAAV-EFS-dSa-VPR mini.

Plasmid

#99686

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLY097-RetroEFS-CD22BBz-PRODH2-WPRE

Plasmid

#192198

Purpose

Retro-CD22CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-CD22CAR-PRODH2 (CD22 Synthetic)

Use

Retroviral; Mammalian expression

Mutation

NA

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11

Plasmid

#192147

Purpose

T cell CRISPR lentiviral vector

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Type

Empty backbone

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP307-pAAV-EFS(No AgeI)-MCS3-pA

Plasmid

#113684

Purpose

EFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-hTSC1-T2A-Bsd

Plasmid

#193199

Purpose

Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

TSC1 (TSC1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DjCas13d-T2A-mCherry-pA

Plasmid

#192499

Purpose

To express DjCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CasRx-T2A-mCherry-pA

Plasmid

#192494

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-hfCas13d-T2A-mCherry-pA

Plasmid

#192497

Purpose

To express hfCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

high fidelity (hf) version of CasRx (hfCas13d)

Use

AAV

Mutation

Yes: GTGgaatacattaccaacGTGGTGtacGTG

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-MCP-3XBFP-NLS

Plasmid

#236372

Purpose

Overexpression of MCP-3XBFPnls in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

MCP-3XBFPnls

Use

Lentiviral

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-Halo-NLS-tDeg

Plasmid

#236370

Purpose

Overexpression of Halo-NLS-tDeg in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

Halo-NLS-tDeg

Use

Lentiviral

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235309

Purpose

ComMAND open-loop circuit regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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