We narrowed to 24,827 results for: Spr;

1. pCRISPR2_IHF_Opt

   Plasmid

   #149395

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Expression

   Bacterial

   Mutation

   IHFprox site is optimized at key positions needed…

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR2_IHF_Mut

   Plasmid

   #149396

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Expression

   Bacterial

   Mutation

   IHFprox site is mutated at key positions needed f…

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR2_motif_scram

   Plasmid

   #149397

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Expression

   Bacterial

   Mutation

   Upstream motif recognized by Cas1-2/3 during spac…

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET51b_hSPR

   Plasmid

   #187111

   Purpose

   Expression of hSPR in bacteria

   Depositor

   Kai Johnsson

   Article

   Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

   Insert

   hSPR
   

   Tags

   Hisx10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR

   Plasmid

   #186276

   Purpose

   Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicrospira shortened CRISPR array
   

   Expression

   Bacterial

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM33_SPSB1_SPRY

   Plasmid

   #178487

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   SPSB1_SPRY (SPSB1 Synthetic, Human)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPRv1_COX4

   Plasmid

   #177983

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting COX4

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting COX4
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti_CRISPRi_sgAR10

   Plasmid

   #167003

   Purpose

   Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   AR Promoter sgRNA 10 (AR Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103_SPRED3_WH1

   Plasmid

   #109899

   Purpose

   Protein expression and purification of SPRED3_WH1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   SPRED3_WH1 (SPRED3 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_SPRED2_WH1

    Plasmid

    #109825

    Purpose

    Protein expression and purification of SPRED2_WH1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    SPRED2_WH1 (SPRED2 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_SPRED1_WH1

    Plasmid

    #109816

    Purpose

    Protein expression and purification of SPRED1_WH1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    SPRED1_WH1 (SPRED1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPRv2_PROX1_1+4

    Plasmid

    #121177

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiCRISPR_Hs_MBD3_ex2

    Plasmid

    #62623

    Purpose

    Encodes gRNA which targets human MBD3

    Depositor

    Paul Wade

    Insert

    MBD3 gRNA (MBD3 Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

14. LentiCRISPR_Hs_MBD3_ex3

    Plasmid

    #62624

    Purpose

    Encodes gRNA which targets human MBD3

    Depositor

    Paul Wade

    Insert

    MBD3 gRNA (MBD3 Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

15. SpRY Cas9

    Plasmid

    #188491

    Purpose

    Expresses FLAG tagged SpRY Cas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR v2-Blast

    Plasmid

    #83480

    Purpose

    Mammalian expression of Cas9 and sgRNA scaffold

    Depositor

    Mohan Babu

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    EF-1α

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCRISPRv2-mCherry

    Plasmid

    #99154

    Purpose

    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

    Depositor

    Agata Smogorzewska

    Inserts

    Cas9
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRISPRoff-v2.1

    Plasmid

    #167981

    Purpose

    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

    Depositor

    Luke Gilbert

    Article

    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

    Insert

    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    

    Tags

    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR-mcBEST

    Plasmid

    #209416

    Purpose

    Plasmid for multiplexed cytosine base editing in streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

    Inserts

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    base editing cassette: PtipA ; sgRNAs: PkasO* an…

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiCRISPR v2 mBAX

    Plasmid

    #129577

    Purpose

    CRISPR deletion of murine BAX

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs murine BAX (Bax Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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