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MG1655-OptoCre-knt-P-R*

Bacterial Strain

#188478

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
MG1655-OptoCre-cat-T172A-P-R

Bacterial Strain

#188480

Purpose

Cre-inducible activation of catT172Afor chloramphenicol resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
pMIG-hH4-AVITEV

Plasmid

#74051

Purpose

retroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Histone-H4 (H4C16 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Promoter

pMSCV-LTRs

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pWJPrP1

Plasmid

#22109

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Mutation

mutations at aa 108 and 111 to make 3F4 epitope (…

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only
pMXs-LNI (with mNgn2x3HA)

Plasmid

#233195

Purpose

Retroviral expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0002

Plasmid

#117543

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + SpCas9-p (no stop codon)(pEPOR0SP0009) + YFP (pICSL50005)+ Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pX335_HR_Prnp_3

Plasmid

#78622

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CDK7 D155A HA (P#812)

Plasmid

#14648

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk7 (CDK7 Human)

Tags

HA

Expression

Mammalian

Mutation

D155A: dominant negative

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pWJPrP5

Plasmid

#22106

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Mutation

mutated codon 177 (D to N)

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-LNI-bGH

Plasmid

#233203

Purpose

AAV expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Lhx3, Isl1 Mouse)

Use

AAV

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-NIL (with mNgn2x3HA)

Plasmid

#233192

Purpose

Retroviral expression of Ngn2x3HA, Isl1, and Lhx3 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mNgn2x3HA-P2A-Isl1-T2A-mLhx3 (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-NLI (with mNgn2x3HA)

Plasmid

#233193

Purpose

Retroviral expression of Ngn2x3HA, Lhx3, and Isl1 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mNgn2x3HA-P2A-mLhx3-T2A-Isl1 (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-LIN (with mNgn2x3HA)

Plasmid

#233194

Purpose

Retroviral expression of Lhx3, Isl1, and Ngn2x3HA for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mLhx3-P2A-Isl1-T2A-mNgn2x3HA (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-ILN (with mNgn2x3HA)

Plasmid

#233196

Purpose

Retroviral expression of Isl1, Lhx3, and Ngn2x3HA for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

Isl1-P2A-mLhx3-T2A-mNgn2x3HA (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-INL (with mNgn2x3HA)

Plasmid

#233197

Purpose

Retroviral expression of Isl1, Ngn2x3HA, and Lhx3 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

Isl1-P2A-mNgn2x3HA-T2A-mLhx3 (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2)_Prnp_SAM2

Plasmid

#78625

Purpose

Encodes sgRNA2.0 targeting 138nt upstream of TSS

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2)_Prnp_SAM1

Plasmid

#78624

Purpose

Encodes sgRNA2.0 targeting 158nt upstream of TSS

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-Brd4 del ET

Plasmid

#21938

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1-1362 only, ET domain deleted

Available Since

Sept. 16, 2009

Availability

Academic Institutions and Nonprofits only
Myc-E-Syt1

Plasmid

#66833

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt1 (ESYT1 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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FW102 OL2-62

Bacterial Strain

#53735

Purpose

Bacterial-two hybrid reporter strain

Depositor

Ann Hochschild

Article

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Bacterial Resistance

Kanamycin and Streptomycin

Species

Escherichia coli

Available Since

June 18, 2014

Availability

Academic Institutions and Nonprofits only