We narrowed to 9,863 results for: 158

1. pOPINK-Cezanne (OTU, aa 53-446)

   Plasmid

   #61581

   Purpose

   Expresses human Cezanne (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   Cezanne (OTUD7B Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-52 and aa 447-843.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT-mMAPT

   Plasmid

   #231584

   Purpose

   Minigene of mouse MAPT containing exon 9, 10, 11 and relevant intronic sequences

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

   Insert

   mouse MAPT (Mapt Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. B-MFF

   Plasmid

   #141158

   Purpose

   Dimerization-dependent fluorescent protein binding partner targeted to outer mitochondrial membrane

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   mitochondrial fission factor (MFF Human)
   

   Use

   Tags

   GB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBLO 62.4

   Plasmid

   #123123

   Purpose

   Human expression vector for deltaproteobacteria CasX and sgRNA

   Depositor

   Benjamin Oakes, Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Inserts

   CasX protein
   CasX sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. IMPT-10316:GABR1

   Plasmid

   #157850

   Purpose

   HA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expression

   Depositor

   Raymond Stevens, Vadim Cherezov

   Article

   Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

   Insert

   GABR1 (GABBR1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBLO 62.5

   Plasmid

   #123124

   Purpose

   Human expression vector for planctomyces CasX and sgRNA

   Depositor

   Benjamin Oakes, Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Inserts

   CasX protein
   CasX sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28-His-MBP-NLS-RfxCas13d-NLS-HA

   Plasmid

   #171586

   Purpose

   Bacterial expression of NLS-RfxCas13d-NLS-HA

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   His-MBP-NLS-RfxCas13d-NLS-HA
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

   Plasmid

   #172501

   Purpose

   Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 full locus with Golden gate acceptor site for spacer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWP1_mHAL

   Plasmid

   #102318

   Purpose

   expression of mouse cDNA of HAL refractory to the guide in plasmid 4

   Depositor

   David Sabatini

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   Hal (Hal Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. G1397 DddAtox-C–SaKKH-Cas9(D10A)

    Plasmid

    #157840

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-DAXX-His

    Plasmid

    #175782

    Purpose

    DAXX protein for bacteria expression system

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIRESneo-Ago2

    Plasmid

    #215841

    Purpose

    Expression of AGO2

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

    Insert

    Human AGO2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5FRT PUR mGSK3A K148A

    Plasmid

    #35158

    Depositor

    Jim Woodgett

    Article

    Doble et al Dev Cell. 2007 Jun;12(6):957-71.

    Insert

    GSK-3α (Gsk3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K148A (dominant negative)

    Promoter

    CMV

    Available since

    May 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pQStrep2-ARPC3

    Plasmid

    #31580

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    Actin related protein 2/3 complex, subunit 3 (ARPC3 Human)
    

    Use

    Tags

    His and StrepII-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFGL1252_TetOFF(Hyg)

    Plasmid

    #118992

    Purpose

    TetOFF (promoter-less) cassette with Hygromycin selection

    Depositor

    Naweed Naqvi

    Article

    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

    Inserts

    tTA+Tnos
    Hygromycin cassette
    Ttub+PtetO
    

    Use

    Fungal expression (in magnaporthe oryzae)

    Tags

    Expression

    Mutation

    Codon optimized for Magnaporthe oryzae

    Promoter

    Aspergillus nidulans TrpC promoter, Ustilago mayd…

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV hSyn FLEx-loxp hM4Di-mCherry

    Plasmid

    #161581

    Purpose

    Expression of hM4Di-mCherry in a cre-dependent fashion

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM4Di-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    hSyn

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBLO ddd64.1

    Plasmid

    #123121

    Purpose

    E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

    Depositor

    Benjamin Oakes, Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    CasX
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Mutated amino acids in catalytic triad to alanine…

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-mCherry2-sgMUC4

    Plasmid

    #198399

    Purpose

    Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    MUC4 sgRNA (MUC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LightR-iCre-miRFP670

    Plasmid

    #162158

    Purpose

    Expresses LightR-iCre-miRFP670 in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    LightR-iCre-miRFP670
    

    Use

    Tags

    NLS and miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PBLO CasX-gRNA

    Plasmid

    #126419

    Purpose

    E. coli vector for CasX gRNA --- Used for In Vitro Transcription

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    casX gRNA
    

    Use

    CRISPR; In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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