Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,339 results for: 290

Showing: 1801 - 1820 of 5339 results

  1. pFsynW SYB2 reporter

    Plasmid
    #197276
    Purpose
    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYB2 and HaloTag (Vamp2 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    N/A
    Promoter
    hSyn (human synapsin I promoter)
    Available Since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197276-plasmid-map-sequence-id-389092

    Enlarge

  2. pEGFP-N1-mCherry-ApoB-TagBFP

    Plasmid
    #129383
    Purpose
    Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP
    Depositor
    Silvestro Conticello
    Article
    Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:
    Insert
    mCherry-ApoB-Tag-BFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available Since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    129383-plasmid-map-sequence-id-254328

    Enlarge

  6. p663-UBC-miniTurbo-V5-NEK1_IDG-K

    Plasmid
    #189871
    Purpose
    Gateway destination clone of NEK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK1 (NEK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189871-plasmid-map-sequence-id-374462

    Enlarge

  9. pCAG-T3-CjCAS9-pA

    Plasmid
    #131543
    Purpose
    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Article
    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    FLAG-SV40NLS, SV40NLS, and polyA
    Expression
    Mammalian
    Mutation
    Human codon-optimized
    Promoter
    CAG, T3
    Available Since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131543-plasmid-map-sequence-id-262486

    Enlarge

  10. pL-SFFV.Reporter.RFP657.PAC

    Plasmid
    #61395
    Purpose
    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.
    Depositor
    Benjamin Ebert
    Article
    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available Since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61395-plasmid-map-sequence-id-101662

    Enlarge

  14. PB-eDIO-EYFP

    Plasmid
    #104388
    Purpose
    PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.
    Depositor
    Volker Busskamp
    Article
    Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10
    Insert
    Enhanced Yellow Fluorescent Protein
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104388-plasmid-map-sequence-id-202374

    Enlarge

  16. pcDNA3.1-mCherry-CyrCR1.miR16

    Plasmid
    #128747
    Purpose
    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.
    Depositor
    David Bartel
    Article
    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do
    Insert
    Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128747-plasmid-map-sequence-id-254135

    Enlarge

  17. pHD157

    Plasmid
    #84033
    Purpose
    Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafish
    Depositor
    Didier Stainier, Daniel Hesselson
    Article
    Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10
    Inserts
    Cre
    Venus
    Use
    Cre/Lox; Zebrafish transgenesis
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Crystalin and fabp10
    Available Since
    Nov. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    84033-plasmid-map-sequence-id-166816

    Enlarge

Showing: 1801 - 1820 of 5339 results