We narrowed to 23,704 results for: RING;

1. pJF3H

   Plasmid

   #63897

   Purpose

   For phage display of cDNA libraries

   Depositor

   Carlos Barbas

   Article

   Barbas et al Cold Spring Harbor, NY: Cold Spring Harb

   Insert

   cDNA cloning region
   

   Use

   Phage display

   Tags

   Fos

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORTMAGE-3

   Plasmid

   #72678

   Purpose

   Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.

   Depositor

   Csaba Pál

   Article

   Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

   Insert

   mutL E32K
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   E32K mutation conferring dominant mutator phenoty…

   Promoter

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRluc-LC3G120A

   Plasmid

   #105003

   Purpose

   Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Mutation

   Glycine 120 mutated to alanine, prevents lipidati…

   Promoter

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUGW-PK-hLC3

   Plasmid

   #61460

   Purpose

   Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

   Depositor

   Isei Tanida

   Article

   Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

   Insert

   PK-hLC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pORTMAGE-4

   Plasmid

   #72679

   Purpose

   Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.

   Depositor

   Csaba Pál

   Article

   Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

   Insert

   mutL E32K
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   E32K mutation conferring dominant mutator phenoty…

   Promoter

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORTMAGE-2

   Plasmid

   #72677

   Purpose

   Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.

   Depositor

   Csaba Pál

   Article

   Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

   Insert

   mutL E32K
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   E32K mutation conferring dominant mutator phenoty…

   Promoter

   pL promoter

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR LAT/Zap70 iLID-Drop

   Plasmid

   #171038

   Purpose

   Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-labeled.

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

   Insert

   LAT (LAT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDual-eGFP(Y203)

   Plasmid

   #63559

   Purpose

   Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(Y203)
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Changed threonine at position 203 with respect to…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Foxf1 HA pShuttle

   Plasmid

   #122120

   Purpose

   Expression of Foxf1 in mammalian cells

   Depositor

   Paul Herring

   Article

   Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

   Insert

   Foxf1 (Foxf1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFR-ET1

    Plasmid

    #44185

    Depositor

    Timothy Springer

    Article

    Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

    Insert

    EGFR-ET1 (EGFR Human)
    

    Use

    Tags

    3C cleavge site, protein C peptide tag, his6, SBP…

    Expression

    Mammalian

    Mutation

    I104V and D254N relative to NP_005219.2

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV CytoBAS

    Plasmid

    #62860

    Purpose

    Fluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

    Depositor

    Stan van de Graaf

    Article

    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

    Insert

    CytoBAS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Raichu-OsRac1 WT

    Plasmid

    #133262

    Purpose

    Biosensor for monitoring activation for rice small GTPase OsRac1

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1 (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV NucleoBAS

    Plasmid

    #62861

    Purpose

    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

    Depositor

    Stan van de Graaf

    Article

    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

    Insert

    NucleoBAS
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. ATP1A1_T804N_hPGK1_EBFP_Donor

    Plasmid

    #187455

    Purpose

    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1-T804N EBFP donor (ATP1A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK1

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Flag1-3HABeta2-mEos2

    Plasmid

    #114187

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Use

    Tags

    3HA, FLAG, and mEOS2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-Ubl-sr1(SIRPT1)

    Plasmid

    #75298

    Purpose

    Expresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)

    Depositor

    Kalle Gehring

    Article

    Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

    Insert

    Human Sacsin 1-339
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. LIC-Z

    Plasmid

    #153543

    Purpose

    perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-hs-3xHA-delta6xNHL-LIN-41

    Plasmid

    #52722

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 593–868

    Promoter

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pL90-Nat-2xHO

    Plasmid

    #139069

    Purpose

    CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Insert

    Two guide RNAs targeting the HO endonuclease
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TDH3

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. EC71167

    Plasmid

    #154066

    Purpose

    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Promoter

    Zea mays Ubiquitin Promoter

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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